|
| Status |
Public on Sep 22, 2007 |
| Title |
PSA-NCAM-positive neuronal precursor cells from adult mouse brain |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
36093 |
| Tag Length |
11 |
| |
|
| Source name |
PSA-NCAM-positive neuronal precursor cells from the adult subventricular zone
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J mice
age: 4-6 week
gender: male
|
| Biomaterial provider |
Harold Cremer, IBDM, Marseille
|
| Treatment protocol |
PSA-NCAM positive cells were isolated from dissected subventricular zone (SVZ) tissue using FACS as described in Pennartz et al., Mol Cell Neurosci. 2004 Apr;25(4):692-706 and PhD thesis "Gene expression analysis of neuronal precursors
from adult mouse brain and differential screen for
neural stem cell markers" , University of Cologne, Germany, http://deposit.ddb.de/cgi-bin/dokserv?idn=973392754
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Lit (Qiagen, Hilden)
|
| |
|
| Description |
No amplification of RNA samples. No cultivation steps, isolated cells were processed immediately.
MicroSAGE Detailed Protocol with minor modifications was performed as described in Pennartz et al., Mol Cell Neurosci. 2004 Apr;25(4):692-706 and PhD thesis "Gene expression analysis of neuronal precursors
from adult mouse brain and differential screen for
neural stem cell markers" , University of Cologne, Germany, http://deposit.ddb.de/cgi-bin/dokserv?idn=973392754
|
| Data processing |
SAGE data analysis was carried out using the Memorec SAGE Analysis Package (Memorec Biotec GmbH, Cologne, Germany).
|
| |
|
| Submission date |
Sep 18, 2007 |
| Last update date |
Nov 19, 2008 |
| Contact name |
Sandra Juliane Pennartz |
| E-mail(s) |
sandrap@miltenyibiotec.de
|
| Organization name |
Miltenyi Biotec GmbH
|
| Department |
Research & Development
|
| Street address |
Friedrich-Ebert-Strasse 68
|
| City |
Bergisch Gladbach |
| ZIP/Postal code |
D-51429 |
| Country |
Germany |
| |
|
| Platform ID |
GPL5885 |
| Series (1) |
| GSE9081 |
Purification of neuronal precursors from the adult mouse brain: a comprehensive gene expression analysis |
|
