As described previously: Nuwaysir EF, et all. 2002. Gene expression analysis using oligonucleotide arrays produced by maskless photolithography. Genome Res. 12:1749-55.
Hybridization protocol
As described previously: Nuwaysir EF, et all. 2002. Gene expression analysis using oligonucleotide arrays produced by maskless photolithography. Genome Res. 12:1749-55.
Scan protocol
with GenePix
Description
Total RNA from RPB1 wild type strain growing at 30C in rich media in day A (N1). Robust Multiarray Average (RMA) using quantile normalization (see: Bolstad B et all. 2003. A comparison of normalization methods for high density oligonucleotide array data based on bias and variance. Bioinformatics 19: 185-193) and gene calls generated using the RMA algorithm (Irizarry RA. et all. 2003. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics, 4:249-64).
