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Sample GSM2308683 Query DataSets for GSM2308683
Status Public on Dec 06, 2016
Title LNCaP_siERRg_vehicle_rep2
Sample type RNA
 
Source name LNCaP cells, siRNA targeted against ERRg, 72h vehicle treatment
Organism Homo sapiens
Characteristics cell line: LNCaP
tissue: prostate cancer cells (immortalized cell line)
Treatment protocol Cells were treated for 72h with R1881 (10nM) or vehicle (EtOH).
Growth protocol Cells were seeded in RPMI media with no phenol red and containing 2% serum deprived from steroids and transfected with siRNA controls or against estrogen-related receptor gamma. 48h later, cell were trypsinized and seeded in 6-well plates for treatment with 10nM R1881 or vehicle (EtOH) for 72h, with fresh medium and treatment added after 48h. Triplicate samples were sent for each conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified using RNeasy mini kit from QIAGEN
Label Cy3
Label protocol Labeling was performed using standard Affymetrix protocol at Génome Québec Innovation Centre (McGill University)
 
Hybridization protocol RNA was hybridized to GeneChip® Human Genome U133 Plus 2.0 (Affymetrix) and quantified at Génome Québec Innovation Centre (McGill University)
Scan protocol Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
Description Sample name: sierrbasal2
Data processing FlexArray10 software was using for data normalization and expression comparison between groups.
 
Submission date Sep 09, 2016
Last update date Dec 06, 2016
Contact name Etienne Audet-Walsh
E-mail(s) etienne.audet-walsh@crchudequebec.ulaval.ca
Organization name Université Laval
Department Molecular Medicine
Street address 2705 boulevard Laurier
City Québec City
State/province QC
ZIP/Postal code G1V 4G2
Country Canada
 
Platform ID GPL10558
Series (1)
GSE86781 Androgen-dependent repression of estrogen-related receptor γ expression mediates metabolic reprogramming in prostate cancer

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
ILMN_1728255 7.08953810557534
ILMN_1846128 7.08430380212369
ILMN_1853167 7.13795157108835
ILMN_1695844 7.0837598667118
ILMN_3243634 7.08499314393662
ILMN_1874323 7.18065349798139
ILMN_1666310 7.12671175147486
ILMN_1757152 7.09678698184559
ILMN_3236052 7.07824820500137
ILMN_1662964 8.57721336697466
ILMN_2043569 7.15320136437044
ILMN_1811364 7.08968776675153
ILMN_1719498 7.2315245730615
ILMN_1763663 7.78987743415963
ILMN_1842320 7.08305584200611
ILMN_2278908 12.7202042112824
ILMN_1777359 7.07834658548189
ILMN_1779373 7.10843292005807
ILMN_1800412 7.4204650393081
ILMN_1812441 7.92372332013832

Total number of rows: 47323

Table truncated, full table size 1381 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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