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Sample GSM2308685 Query DataSets for GSM2308685
Status Public on Dec 06, 2016
Title LNCaP_siERRg_r1881_rep1
Sample type RNA
 
Source name LNCaP cells, siRNA targeted against ERRg, 72h R1881 treatment
Organism Homo sapiens
Characteristics cell line: LNCaP
tissue: prostate cancer cells (immortalized cell line)
Treatment protocol Cells were treated for 72h with R1881 (10nM) or vehicle (EtOH).
Growth protocol Cells were seeded in RPMI media with no phenol red and containing 2% serum deprived from steroids and transfected with siRNA controls or against estrogen-related receptor gamma. 48h later, cell were trypsinized and seeded in 6-well plates for treatment with 10nM R1881 or vehicle (EtOH) for 72h, with fresh medium and treatment added after 48h. Triplicate samples were sent for each conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified using RNeasy mini kit from QIAGEN
Label Cy3
Label protocol Labeling was performed using standard Affymetrix protocol at Génome Québec Innovation Centre (McGill University)
 
Hybridization protocol RNA was hybridized to GeneChip® Human Genome U133 Plus 2.0 (Affymetrix) and quantified at Génome Québec Innovation Centre (McGill University)
Scan protocol Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
Description Sample name: sierrr18811
Data processing FlexArray10 software was using for data normalization and expression comparison between groups.
 
Submission date Sep 09, 2016
Last update date Dec 06, 2016
Contact name Etienne Audet-Walsh
E-mail(s) etienne.audet-walsh@crchudequebec.ulaval.ca
Organization name Université Laval
Department Molecular Medicine
Street address 2705 boulevard Laurier
City Québec City
State/province QC
ZIP/Postal code G1V 4G2
Country Canada
 
Platform ID GPL10558
Series (1)
GSE86781 Androgen-dependent repression of estrogen-related receptor γ expression mediates metabolic reprogramming in prostate cancer

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
ILMN_1728255 7.09480303707009
ILMN_1846128 7.07695179833929
ILMN_1853167 7.14268438648357
ILMN_1695844 7.09886396084514
ILMN_3243634 7.07807774299977
ILMN_1874323 7.17525185681598
ILMN_1666310 7.08542718006655
ILMN_1757152 7.10035479908939
ILMN_3236052 7.08241000566589
ILMN_1662964 8.22207359226023
ILMN_2043569 7.11180091742131
ILMN_1811364 7.12533409568658
ILMN_1719498 7.17102241234363
ILMN_1763663 7.96721484550548
ILMN_1842320 7.0798147797894
ILMN_2278908 13.0730441544015
ILMN_1777359 7.09441411194306
ILMN_1779373 7.14105271815091
ILMN_1800412 7.21663654928509
ILMN_1812441 7.67096718926089

Total number of rows: 47323

Table truncated, full table size 1381 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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