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Status |
Public on Dec 06, 2016 |
Title |
LNCaP_siERRg_r1881_rep1 |
Sample type |
RNA |
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Source name |
LNCaP cells, siRNA targeted against ERRg, 72h R1881 treatment
|
Organism |
Homo sapiens |
Characteristics |
cell line: LNCaP tissue: prostate cancer cells (immortalized cell line)
|
Treatment protocol |
Cells were treated for 72h with R1881 (10nM) or vehicle (EtOH).
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Growth protocol |
Cells were seeded in RPMI media with no phenol red and containing 2% serum deprived from steroids and transfected with siRNA controls or against estrogen-related receptor gamma. 48h later, cell were trypsinized and seeded in 6-well plates for treatment with 10nM R1881 or vehicle (EtOH) for 72h, with fresh medium and treatment added after 48h. Triplicate samples were sent for each conditions.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was purified using RNeasy mini kit from QIAGEN
|
Label |
Cy3
|
Label protocol |
Labeling was performed using standard Affymetrix protocol at Génome Québec Innovation Centre (McGill University)
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Hybridization protocol |
RNA was hybridized to GeneChip® Human Genome U133 Plus 2.0 (Affymetrix) and quantified at Génome Québec Innovation Centre (McGill University)
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Scan protocol |
Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
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Description |
Sample name: sierrr18811
|
Data processing |
FlexArray10 software was using for data normalization and expression comparison between groups.
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Submission date |
Sep 09, 2016 |
Last update date |
Dec 06, 2016 |
Contact name |
Etienne Audet-Walsh |
E-mail(s) |
etienne.audet-walsh@crchudequebec.ulaval.ca
|
Organization name |
Université Laval
|
Department |
Molecular Medicine
|
Street address |
2705 boulevard Laurier
|
City |
Québec City |
State/province |
QC |
ZIP/Postal code |
G1V 4G2 |
Country |
Canada |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE86781 |
Androgen-dependent repression of estrogen-related receptor γ expression mediates metabolic reprogramming in prostate cancer |
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