NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2308687 Query DataSets for GSM2308687
Status Public on Dec 06, 2016
Title LNCaP_siERRg_r1881_rep3
Sample type RNA
 
Source name LNCaP cells, siRNA targeted against ERRg, 72h R1881 treatment
Organism Homo sapiens
Characteristics cell line: LNCaP
tissue: prostate cancer cells (immortalized cell line)
Treatment protocol Cells were treated for 72h with R1881 (10nM) or vehicle (EtOH).
Growth protocol Cells were seeded in RPMI media with no phenol red and containing 2% serum deprived from steroids and transfected with siRNA controls or against estrogen-related receptor gamma. 48h later, cell were trypsinized and seeded in 6-well plates for treatment with 10nM R1881 or vehicle (EtOH) for 72h, with fresh medium and treatment added after 48h. Triplicate samples were sent for each conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified using RNeasy mini kit from QIAGEN
Label Cy3
Label protocol Labeling was performed using standard Affymetrix protocol at Génome Québec Innovation Centre (McGill University)
 
Hybridization protocol RNA was hybridized to GeneChip® Human Genome U133 Plus 2.0 (Affymetrix) and quantified at Génome Québec Innovation Centre (McGill University)
Scan protocol Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
Description Sample name: sierrr18813
Data processing FlexArray10 software was using for data normalization and expression comparison between groups.
 
Submission date Sep 09, 2016
Last update date Dec 06, 2016
Contact name Etienne Audet-Walsh
E-mail(s) etienne.audet-walsh@crchudequebec.ulaval.ca
Organization name Université Laval
Department Molecular Medicine
Street address 2705 boulevard Laurier
City Québec City
State/province QC
ZIP/Postal code G1V 4G2
Country Canada
 
Platform ID GPL10558
Series (1)
GSE86781 Androgen-dependent repression of estrogen-related receptor γ expression mediates metabolic reprogramming in prostate cancer

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
ILMN_1728255 7.07979910231951
ILMN_1846128 7.08215842367991
ILMN_1853167 7.12329530118699
ILMN_1695844 7.08115695251581
ILMN_3243634 7.08937712136092
ILMN_1874323 7.09938163297898
ILMN_1666310 7.08088395784655
ILMN_1757152 7.10908703806184
ILMN_3236052 7.07937709095631
ILMN_1662964 8.4140318137888
ILMN_2043569 7.0882875322841
ILMN_1811364 7.16339441709396
ILMN_1719498 7.17847362009501
ILMN_1763663 7.88420179715487
ILMN_1842320 7.08046314209297
ILMN_2278908 13.0078324272893
ILMN_1777359 7.08429691970532
ILMN_1779373 7.11644430513289
ILMN_1800412 7.15980398409337
ILMN_1812441 7.60534080260742

Total number of rows: 47323

Table truncated, full table size 1381 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap