|
| Status |
Public on Sep 08, 2017 |
| Title |
small RNAs produced by Dicer-2 [DHelicase] in vitro |
| Sample type |
SRA |
| |
|
| Source name |
104 bp dsRNA
|
| Organism |
synthetic construct |
| Characteristics |
proteins: Dicer-2[DHelicase]
sample type: in vitro
|
| Extracted molecule |
other |
| Extraction protocol |
Female fly heads were hand-dissected and RNAs were prepared using miRVana.
Libraries were prepared as previously described (Fukunaga et al, 2014 and Han et al, 2015).
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
small RNAs produced from 104 bp dsRNA in vitro
The sequences of strands 1 and 2 of the 104 bp dsRNAs are: 5'-GGGCCACAAGUUCAGCGUGUCCGGCGAGGGCGAGGGCGAUGCCACCUACGGCAAGCUGACCCUGAAGUUCAUCUGCACCACCGGCAAGCUGCCCGUGCCCUGGCCC-3' and 5'-GCCAGGGCACGGGCAGCUUGCCGGUGGUGCAGAUGAACUUCAGGGUCAGCUUGCCGUAGGUGGCAUCGCCCUCGCCCUCGCCGGACACGCUGAACUUGUGGCCCAA-3'. These two strands make 104 bp dsRNA with 3' 2-nt overhang at both ends.
|
| Data processing |
Basecalls performed using bcl2fastq version 2.16
Sequenced reads were trimmed for adaptor sequence.
Genome_build: dm3
Supplementary_files_format_and_content: [.txt] Tab-delimited text file. Length of the read, Number of the read, Sequence of the read.
|
| |
|
| Submission date |
Sep 14, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ryuya Fukunaga |
| E-mail(s) |
fukunaga@jhmi.edu
|
| Phone |
4109553790
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Department of Biological Chemistry
|
| Street address |
725 N. Wolfe St. 521A Physiology Bldg
|
| City |
BALTIMORE |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL21616 |
| Series (1) |
| GSE94803 |
small RNAs produced in the mutant Dicer-2 transgenic flies |
|
| Relations |
| BioSample |
SAMN05771107 |
| SRA |
SRX2606824 |
