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Sample GSM231087 Query DataSets for GSM231087
Status Public on Sep 26, 2007
Title Animal 1 hepatectomy sham surgery 1h
Sample type RNA
Channel 1
Source name Reference RNA
Organism synthetic construct
Characteristics Vector RNA. Hybridizes to all spots evenly
Extracted molecule total RNA
Extraction protocol RT on empty pT7T3Pac vector
Label Cy3
Label protocol indirect labeling protocol
Channel 2
Source name Animal 1 hepatectomy sham surgery 1h
Organism Rattus norvegicus
Characteristics Strain:Sprague-Dawley, Gender:Male
Liver removed 1 h after hepatectomy sham surgery.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.
Label Cy5
Label protocol indirect labeling protocol
Hybridization protocol Reference and experimental probes were combined and competitively hybridized to microarrays for 18 hours at 37C.
Scan protocol Slides were scanned using ScanArray 5000 fluorescent scanner (GSI Lumonics, Billerica, MA). The resulting images were quantified using ScanArray Express v2.2 software with the Adaptive Threshold segmentation (GSI Lumonics, Billerica, MA).
Description To analyze the effects of surgical stress on gene expression levels in rat liver by performing sham surgery on the liver of rats and comparing the livers to reference RNA.
Data processing Raw quantitated array data was normalized using the print-tip lowess and scale normalization algorithms
Submission date Sep 23, 2007
Last update date Aug 14, 2011
Contact name Egle Juskeviciute
Organization name Thomas Jefferson University
Department Pathology
Street address 1020 Locust st. JAH 527
City Philadelphia
State/province PA
ZIP/Postal code 19107
Country USA
Platform ID GPL5051
Series (1)
GSE9137 Hepatectomy sham surgery 1h

Data table header descriptions
VALUE normalized Cy5/Cy3

Data table
1 2.893373301
2 2.871248919
3 0.053580283
4 0.083338096
5 0.753696679
6 0.641963256
7 -2.131482473
8 -2.197830501
9 1.063546743
10 0.829603775
11 0.634264183
12 0.174925661
13 2.707162496
14 2.790104326
15 1.773059212
16 2.079487024
17 -2.071478951
18 -2.356121374

Total number of rows: 18168

Table truncated, full table size 314 Kbytes.

Supplementary file Size Download File type/resource
GSM231087.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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