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Sample GSM231089 Query DataSets for GSM231089
Status Public on Sep 26, 2007
Title Animal 3 hepatectomy sham surgery 1h
Sample type RNA
Channel 1
Source name Reference RNA
Organism synthetic construct
Characteristics Vector RNA. Hybridizes to all spots evenly
Extracted molecule total RNA
Extraction protocol RT on empty pT7T3Pac vector
Label Cy3
Label protocol indirect labeling protocol
Channel 2
Source name Animal 3 hepatectomy sham surgery 1h
Organism Rattus norvegicus
Characteristics Strain:Sprague-Dawley, Gender:Male
Liver removed 1 h after hepatectomy sham surgery.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.
Label Cy5
Label protocol indirect labeling protocol
Hybridization protocol Reference and experimental probes were combined and competitively hybridized to microarrays for 18 hours at 37C.
Scan protocol Slides were scanned using ScanArray 5000 fluorescent scanner (GSI Lumonics, Billerica, MA). The resulting images were quantified using ScanArray Express v2.2 software with the Adaptive Threshold segmentation (GSI Lumonics, Billerica, MA).
Description To analyze the effects of surgical stress on gene expression levels in rat liver by performing sham surgery on the liver of rats and comparing the livers to reference RNA.
Data processing Raw quantitated array data was normalized using the print-tip lowess and scale normalization algorithms
Submission date Sep 23, 2007
Last update date Aug 14, 2011
Contact name Egle Juskeviciute
Organization name Thomas Jefferson University
Department Pathology
Street address 1020 Locust st. JAH 527
City Philadelphia
State/province PA
ZIP/Postal code 19107
Country USA
Platform ID GPL5051
Series (1)
GSE9137 Hepatectomy sham surgery 1h

Data table header descriptions
VALUE normalized Cy5/Cy3

Data table
1 2.732700008
2 2.76552171
3 -0.680982806
4 -0.623575886
5 0.369402856
6 0.582775735
7 -1.913036069
8 -1.973008841
9 -0.270171721
10 0.966788454
11 0.485527108
12 0.5988057
13 2.211327376
14 2.828488261
15 1.756612886
16 1.922471074
17 -0.522389076
18 -1.177985324
19 0.375418432
20 0.503725354

Total number of rows: 18168

Table truncated, full table size 314 Kbytes.

Supplementary file Size Download File type/resource
GSM231089.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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