|
| Status |
Public on Sep 26, 2007 |
| Title |
Animal 3 hepatectomy sham surgery 1h |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Reference RNA
|
| Organism |
synthetic construct |
| Characteristics |
Vector RNA. Hybridizes to all spots evenly
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RT on empty pT7T3Pac vector
|
| Label |
Cy3
|
| Label protocol |
indirect labeling protocol
|
| |
|
| Channel 2 |
| Source name |
Animal 3 hepatectomy sham surgery 1h
|
| Organism |
Rattus norvegicus |
| Characteristics |
Strain:Sprague-Dawley, Gender:Male
Liver removed 1 h after hepatectomy sham surgery.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.
|
| Label |
Cy5
|
| Label protocol |
indirect labeling protocol
|
| |
|
| |
| Hybridization protocol |
Reference and experimental probes were combined and competitively hybridized to microarrays for 18 hours at 37C.
|
| Scan protocol |
Slides were scanned using ScanArray 5000 fluorescent scanner (GSI Lumonics, Billerica, MA). The resulting images were quantified using ScanArray Express v2.2 software with the Adaptive Threshold segmentation (GSI Lumonics, Billerica, MA).
|
| Description |
To analyze the effects of surgical stress on gene expression levels in rat liver by performing sham surgery on the liver of rats and comparing the livers to reference RNA.
|
| Data processing |
Raw quantitated array data was normalized using the print-tip lowess and scale normalization algorithms
|
| |
|
| Submission date |
Sep 23, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Egle Juskeviciute |
| Organization name |
Thomas Jefferson University
|
| Department |
Pathology
|
| Street address |
1020 Locust st. JAH 527
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
| |
|
| Platform ID |
GPL5051 |
| Series (1) |
| GSE9137 |
Hepatectomy sham surgery 1h |
|
