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Sample GSM231270 Query DataSets for GSM231270
Status Public on Sep 26, 2007
Title SHDO195
Sample type RNA
 
Channel 1
Source name Pool
Organism Homo sapiens
Characteristics Pool of human subcutaneous adipose tissue
Extracted molecule total RNA
Extraction protocol Total RNA from scWAT was prepared using the RNeasy RNA Mini Kit (Qiagen, Courtaboeuf, France).
Label Cy3
Label protocol One microgram of total RNA from each sample preparation was amplified using the MessageAmp RNA kit (Ambion, Austin, TX), and 3 µg aRNA was Cy-dye labeled using the CyScribe first-strand cDNA labeling kit (Amersham Biosciences, Orsay, France) (available at http://cmgm.stanford.edu/pbrown/protocols/index).
 
Channel 2
Source name Obese
Organism Homo sapiens
Characteristics Obese subcutaneous adipose tissue (DA)
Extracted molecule total RNA
Extraction protocol Total RNA from scWAT was prepared using the RNeasy RNA Mini Kit (Qiagen, Courtaboeuf, France).
Label Cy5
Label protocol One microgram of total RNA from each sample preparation was amplified using the MessageAmp RNA kit (Ambion, Austin, TX), and 3 µg aRNA was Cy-dye labeled using the CyScribe first-strand cDNA labeling kit (Amersham Biosciences, Orsay, France) (available at http://cmgm.stanford.edu/pbrown/protocols/index).
 
 
Hybridization protocol The labeled cDNA mixtures were hybridized according to the protocol described at http://cmgm.stanford.edu/pbrown/protocols/index
Scan protocol The arrays were immediately scanned using a GenePix 4000A confocal Scanner (Axon Instruments). Images were analyzed using GenePix pro 3 software.
Description We analyzed the gene expression profiles of subcutaneous white adipose tissue from 29 obese subjects during very low calorie diet (VLCD) using cDNA microarray. The patterns of expression were compared with that of 17 non-obese subjects.
Data processing After scanning the arrays, the images were analyzed and ratio intensities were filtered and normalized using Stanford Microarray Database Online resources (http://genome-www5.stanford.edu/).The VALUE field represents the log(base2) of the print-tip loess normalized R/G ratio (mean).
 
Submission date Sep 24, 2007
Last update date Aug 14, 2011
Contact name Corneliu Henegar
E-mail(s) corneliu@henegar.info, flavien.jacques@upmc.fr
Organization name Institut national de la santé et de la recherche médicale
Department U872 Eq7
Lab Eq7 Nutriomique
Street address 15 Rue de l'Ecole de Medecine
City Paris
State/province Ile de France
ZIP/Postal code 75005
Country France
 
Platform ID GPL5925
Series (2)
GSE9153 Expression profiling of human adipose tissue in obese subjects
GSE9157 Expression profiling of human adipose tissue in obese and lean subjects and in various clinical conditions

Data table header descriptions
ID_REF
VALUE log(base2) of print-tip loess normalized R/G ratio (mean)

Data table
ID_REF VALUE
1 -0.232
2 -0.706
3 0.048
4 -0.728
5 1.207
6 -0.359
7 1.388
8 0.023
9 0.059
10 0.097
11 0.205
12 0.65
13 0.085
14 -0.047
15 0.548
16 -0.242
17 0.305
18 0.373
19 -0.302
20 -0.686

Total number of rows: 43104

Table truncated, full table size 509 Kbytes.




Supplementary file Size Download File type/resource
GSM231270.gpr.gz 2.8 Mb (ftp)(http) GPR
Processed data included within Sample table

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