Protocol 1 and 2 are new DNA extraction protocols, protocol 3 is modified Qiagen extraction protocol and 4 is Qiagen DNA extraction protocol.
Label
biotin
Label protocol
Biotinylated DNA were prepared according to the standard Affymetrix protocol from 90 ug total DNA (250K StyI Mapping Asssay Technical Manual, 2006, Affymetrix).
Hybridization protocol
Following fragmentation, 90 ug of DNA were hybridized for 16 hr at 49C on 250K StyI mapping Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol
GeneChips were scanned using the Affymetrix Scanner 7000.
Description
peripheral blood
Data processing
The data were analyzed with CNAG v2.0, AsCNAR and CNATv4.0.1.