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Sample GSM231997 Query DataSets for GSM231997
Status Public on Jul 01, 2008
Title LNCAp control, biological rep 2
Sample type RNA
 
Source name LNCaP cells, 48 hours post control transfection
Organism Homo sapiens
Characteristics LNCap cells
Treatment protocol Cells were transiently transfected with 100nM siRNA or 4ug vector DNA.
Growth protocol LNCaP cells grown in T-medium containing 10% FBS at 37C and 5% CO2.
Extracted molecule total RNA
Extraction protocol Rneasy extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 ug total RNA.
 
Hybridization protocol cRNA probes were hybridized overnight to the Human Genome U133 GeneChip set. GeneChips were washed in the Affymetrix GeneChip Fluidics WorkStation.
Scan protocol GeneChips were scanned according to manufacturer's protocol.
Description Gene expression data from LNCaP cells with control transfection
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) to filter genes that were called present in fewer that 50% of samples.
 
Submission date Sep 27, 2007
Last update date Aug 14, 2011
Contact name Colleen D McCabe
Organization name Emory University
Street address 615 Michael St
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
 
Platform ID GPL96
Series (1)
GSE9182 Expression data from overexpression and knockdown of HOXC6 in LNCaP cells

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 411.9 P 0.001102
AFFX-BioB-M_at 814 P 0.000972
AFFX-BioB-3_at 415.8 P 0.000081
AFFX-BioC-5_at 1410.5 P 0.000052
AFFX-BioC-3_at 971.6 P 0.000052
AFFX-BioDn-5_at 1008.4 P 0.00006
AFFX-BioDn-3_at 5678.4 P 0.00007
AFFX-CreX-5_at 11401.7 P 0.000044
AFFX-CreX-3_at 18763.4 P 0.000044
AFFX-DapX-5_at 30.1 A 0.195266
AFFX-DapX-M_at 28.7 A 0.368438
AFFX-DapX-3_at 3.7 A 0.995002
AFFX-LysX-5_at 9.6 A 0.60308
AFFX-LysX-M_at 29 A 0.51489
AFFX-LysX-3_at 19.1 A 0.354453
AFFX-PheX-5_at 3.3 A 0.883887
AFFX-PheX-M_at 8.4 A 0.891021
AFFX-PheX-3_at 51.7 A 0.185131
AFFX-ThrX-5_at 13.8 A 0.645547
AFFX-ThrX-M_at 6.7 A 0.804734

Total number of rows: 22283

Table truncated, full table size 583 Kbytes.




Supplementary file Size Download File type/resource
GSM231997.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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