|
| Status |
Public on Jul 01, 2008 |
| Title |
LNCaP HOXC6 siRNA, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP cells, 48 hours post siRNA knockdown of HOXC6 transfection
|
| Organism |
Homo sapiens |
| Characteristics |
LNCaP cells after siRNA knockdown of HOXC6 transfection
|
| Treatment protocol |
Cells were transiently transfected with 100nM siRNA or 4ug vector DNA.
|
| Growth protocol |
LNCaP cells grown in T-medium containing 10% FBS at 37C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 ug total RNA.
|
| |
|
| Hybridization protocol |
cRNA probes were hybridized overnight to the Human Genome U133 GeneChip set. GeneChips were washed in the Affymetrix GeneChip Fluidics WorkStation.
|
| Scan protocol |
GeneChips were scanned according to manufacturer's protocol.
|
| Description |
Gene expression data from LNCaP cells with HOXC6 siRNA knockdown
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) to filter genes that were called present in fewer that 50% of samples.
|
| |
|
| Submission date |
Sep 27, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Colleen D McCabe |
| Organization name |
Emory University
|
| Street address |
615 Michael St
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE9182 |
Expression data from overexpression and knockdown of HOXC6 in LNCaP cells |
|
