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Sample GSM2359122 Query DataSets for GSM2359122
Status Public on Oct 17, 2017
Title Cadaver6
Sample type RNA
 
Source name Cadaver: Human Islets LCM
Organism Homo sapiens
Characteristics tissue: pancreas
cell type: Islets LCM
age: 54
gender: M
bmi: 35
Growth protocol LCM samples were obtained from selected beta cell rich autofluorescent areas of islets or from generalized non-selected areas LCM of islet tissue.
Extracted molecule total RNA
Extraction protocol PicoPure, Dnase treatment, RiboAmp HS RNA Amplification Kit
Label biotin
Label protocol Simultaneous cRNA synthesis and biotin labeling via 3' IVT protocol
 
Hybridization protocol 10ug cRNA was used to make 250ul hyb cocktail, of which 200ul were injected into GeneChip X3P arrays and hybridized for 16 hours at 45C. Arrays were washed and stained using Affymetrix Fluidics Station 450 with user-prepared reagents.
Scan protocol Microarrays were scanned using the GeneChip Scanner 3000 system from Affymetrix.
Data processing Data were normalized using RMA and the Affymetrix Command Console module.
 
Submission date Oct 24, 2016
Last update date Oct 19, 2017
Contact name Hui Pan
E-mail(s) Hui.Pan@joslin.harvard.edu
Organization name Joslin Diabetes Center
Department Bioinformatics and Biostatistics Core
Street address 1 Joslin Place
City Boston
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1352
Series (1)
GSE89120 Evidence of stress in beta cells obtained with laser capture microdisssection from pancreases of brain-dead donor

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1053_3p_at 3.669184
117_3p_at 4.08092
1494_3p_f_at 5.036997
1552275_3p_s_at 3.670536
1552281_3p_at 6.44491
1552296_3p_at 3.20668
1552302_3p_at 2.059227
1552311_3p_a_at 5.783033
1552321_3p_a_at 2.345997
1552340_3p_at 3.053476
1552343_3p_s_at 3.357929
1552354_3p_at 3.073641
1552476_3p_s_at 4.5009
1552480_3p_s_at 1.726755
1552504_3p_a_at 4.521833
1552518_3p_s_at 1.806514
1552528_3p_at 5.164138
1552535_3p_at 4.599692
1552566_3p_at 3.772694
1552575_3p_a_at 3.811245

Total number of rows: 61359

Table truncated, full table size 1712 Kbytes.




Supplementary file Size Download File type/resource
GSM2359122_Jos4326.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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