NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2359126 Query DataSets for GSM2359126
Status Public on Oct 17, 2017
Title Surgical6
Sample type RNA
 
Source name Surgical: Human Islets LCM
Organism Homo sapiens
Characteristics tissue: pancreas
cell type: Islets LCM
age: 57
gender: F
bmi: 24
Growth protocol LCM samples were obtained from selected beta cell rich autofluorescent areas of islets or from generalized non-selected areas LCM of islet tissue.
Extracted molecule total RNA
Extraction protocol PicoPure, Dnase treatment, RiboAmp HS RNA Amplification Kit
Label biotin
Label protocol Simultaneous cRNA synthesis and biotin labeling via 3' IVT protocol
 
Hybridization protocol 10ug cRNA was used to make 250ul hyb cocktail, of which 200ul were injected into GeneChip X3P arrays and hybridized for 16 hours at 45C. Arrays were washed and stained using Affymetrix Fluidics Station 450 with user-prepared reagents.
Scan protocol Microarrays were scanned using the GeneChip Scanner 3000 system from Affymetrix.
Data processing Data were normalized using RMA and the Affymetrix Command Console module.
 
Submission date Oct 24, 2016
Last update date Oct 19, 2017
Contact name Hui Pan
E-mail(s) Hui.Pan@joslin.harvard.edu
Organization name Joslin Diabetes Center
Department Bioinformatics and Biostatistics Core
Street address 1 Joslin Place
City Boston
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1352
Series (1)
GSE89120 Evidence of stress in beta cells obtained with laser capture microdisssection from pancreases of brain-dead donor

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1053_3p_at 3.808583
117_3p_at 4.299824
1494_3p_f_at 4.975916
1552275_3p_s_at 3.96572
1552281_3p_at 6.476546
1552296_3p_at 3.484441
1552302_3p_at 1.976359
1552311_3p_a_at 6.12165
1552321_3p_a_at 2.393142
1552340_3p_at 3.144184
1552343_3p_s_at 3.679143
1552354_3p_at 2.931913
1552476_3p_s_at 4.444834
1552480_3p_s_at 1.593718
1552504_3p_a_at 4.934705
1552518_3p_s_at 1.682965
1552528_3p_at 5.11357
1552535_3p_at 4.891223
1552566_3p_at 3.453687
1552575_3p_a_at 3.834649

Total number of rows: 61359

Table truncated, full table size 1712 Kbytes.




Supplementary file Size Download File type/resource
GSM2359126_Jos4348.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap