|
| Status |
Public on Mar 30, 2017 |
| Title |
0-2 hr embryo Rnase R |
| Sample type |
SRA |
| |
|
| Source name |
0-2 hr embryo Rnase R
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: y w
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extraction in accordance to Trizol protocol.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
rRNA-depleted Rnase treated total RNA
|
| Data processing |
Sequenced and aligned reads were passed through a Perl filtering algorithm to determine if they were internal to an intron or overlapped with the 5' or 3' junction of each intron in the Drosophila melanogaster (dm3) genome. Each intron was indexed and reads that aligned to the index were parsed and counted to determine molecule size. Sequence information was then extracted for each putative molecule, and pairwise aligned using Blast (43). Positive Blast hits were then aligned with Clustalw2 to determine actual sequence alignment for further downstream analysis (44).
Genome_build: dm3
|
| |
|
| Submission date |
Nov 03, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jun Wei Pek |
| E-mail(s) |
junwei@tll.org.sg
|
| Phone |
+6568727818
|
| Organization name |
Temasek Life Sciences Laboratory
|
| Street address |
1 Research Link National University of Singapore
|
| City |
Singapore |
| ZIP/Postal code |
117604 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL13304 |
| Series (1) |
| GSE89496 |
RNA-seq of Drosophila 0-2 hr embryos Rnase R |
|
| Relations |
| Reanalyzed by |
GSM3282206 |
| BioSample |
SAMN05977311 |
| SRA |
SRX2324002 |
