NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2389463 Query DataSets for GSM2389463
Status Public on Nov 01, 2022
Title AP5.24hr.1
Sample type RNA
 
Channel 1
Source name RNA purified from cultured neurons
Organism Mus musculus
Characteristics tissue: neurons
Growth protocol DIV 21 dissociated mouse hippocampal neurons in 6 well plates were treated by Vehicle, 100 μM AP5, 100 nM NVP-AAM077 or 1 μM Ro25-4891 for 24 hours in a cell culture incubator    
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using RNeasy plus kit (Qiagen)
Label Cy5
Label protocol Briefly, total RNA sample was converted to double-stranded cDNA and then to Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. The labeled cRNA was purified using RNeasy mini kit (Qiagen, San Diego, CA). cRNA yield and Cy-dye incorporation was determined using ND-1000 spectrophotometer (Thermo Scientific)
 
Channel 2
Source name universal mouse reference (Stratagene, La Jolla, CA)
Organism Mus musculus
Characteristics tissue: universal mouse reference
Growth protocol DIV 21 dissociated mouse hippocampal neurons in 6 well plates were treated by Vehicle, 100 μM AP5, 100 nM NVP-AAM077 or 1 μM Ro25-4891 for 24 hours in a cell culture incubator    
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using RNeasy plus kit (Qiagen)
Label Cy3
Label protocol Briefly, total RNA sample was converted to double-stranded cDNA and then to Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. The labeled cRNA was purified using RNeasy mini kit (Qiagen, San Diego, CA). cRNA yield and Cy-dye incorporation was determined using ND-1000 spectrophotometer (Thermo Scientific)
 
 
Hybridization protocol 750 ng of the labeled cRNA was fragmented and hybridized to arrays as described in manufacturer’s hybridization kit
Scan protocol Scanned on an Agilent G2565AA scanner.
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
Description AP5.24hr.1
Data processing  Agilent’s Feature Extraction software 11.0 was used to analyze acquired array images
 
Submission date Nov 14, 2016
Last update date Nov 01, 2022
Contact name Josh Kaminker
Organization name Genentech
Department Bioinformatics and Computational Biology
Street address 1 DNA Way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
 
Platform ID GPL7202
Series (1)
GSE89802 Neuronal activity stimulates astrocyte proliferation through NMDA receptor-dependent induction of Prostaglandin-Endoperoxide Synthase 2

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
A_52_P298165 0.019694327
A_52_P96028 0.101819031
A_52_P413492 -2.21894166
A_51_P351598 -0.238556729
A_52_P700056 -2.453716779
A_51_P199778 0.159392626
A_52_P660466 0.119742547
A_52_P62011 1.174356243
A_51_P134184 0.167266994
A_52_P265820 0.20219315
A_52_P27056 0.083008556
A_52_P61833 0.252256231
A_51_P375631 0.552943847
A_51_P432011 -0.410077552
A_52_P160057 3.054267854
A_52_P633785 0.982733963
A_51_P204237 -0.013133405
A_52_P1053160 -0.238457207
A_52_P677891 3.398583136
A_52_P352541 2.056247277

Total number of rows: 20674

Table truncated, full table size 510 Kbytes.




Supplementary file Size Download File type/resource
GSM2389463_Agilent_251486827963_S01_GE2_107_Sep09_1_1.txt.gz 14.4 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap