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Sample GSM2389465 Query DataSets for GSM2389465
Status Public on Nov 01, 2022
Title AP5.24hr.3
Sample type RNA
 
Channel 1
Source name RNA purified from cultured neurons
Organism Mus musculus
Characteristics tissue: neurons
Growth protocol DIV 21 dissociated mouse hippocampal neurons in 6 well plates were treated by Vehicle, 100 μM AP5, 100 nM NVP-AAM077 or 1 μM Ro25-4891 for 24 hours in a cell culture incubator    
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using RNeasy plus kit (Qiagen)
Label Cy5
Label protocol Briefly, total RNA sample was converted to double-stranded cDNA and then to Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. The labeled cRNA was purified using RNeasy mini kit (Qiagen, San Diego, CA). cRNA yield and Cy-dye incorporation was determined using ND-1000 spectrophotometer (Thermo Scientific)
 
Channel 2
Source name universal mouse reference (Stratagene, La Jolla, CA)
Organism Mus musculus
Characteristics tissue: universal mouse reference
Growth protocol DIV 21 dissociated mouse hippocampal neurons in 6 well plates were treated by Vehicle, 100 μM AP5, 100 nM NVP-AAM077 or 1 μM Ro25-4891 for 24 hours in a cell culture incubator    
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using RNeasy plus kit (Qiagen)
Label Cy3
Label protocol Briefly, total RNA sample was converted to double-stranded cDNA and then to Cy-dye labeled cRNA using Agilent’s Quick Amp Labeling Kit. The labeled cRNA was purified using RNeasy mini kit (Qiagen, San Diego, CA). cRNA yield and Cy-dye incorporation was determined using ND-1000 spectrophotometer (Thermo Scientific)
 
 
Hybridization protocol 750 ng of the labeled cRNA was fragmented and hybridized to arrays as described in manufacturer’s hybridization kit
Scan protocol Scanned on an Agilent G2565AA scanner.
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
Description AP5.24hr.3
Data processing  Agilent’s Feature Extraction software 11.0 was used to analyze acquired array images
 
Submission date Nov 14, 2016
Last update date Nov 01, 2022
Contact name Josh Kaminker
Organization name Genentech
Department Bioinformatics and Computational Biology
Street address 1 DNA Way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
 
Platform ID GPL7202
Series (1)
GSE89802 Neuronal activity stimulates astrocyte proliferation through NMDA receptor-dependent induction of Prostaglandin-Endoperoxide Synthase 2

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
A_52_P298165 -0.074188143
A_52_P96028 0.11084176
A_52_P413492 -2.233373291
A_51_P351598 -0.121236004
A_52_P700056 -2.642005263
A_51_P199778 0.412566194
A_52_P660466 0.126823938
A_52_P62011 1.182046403
A_51_P134184 -0.063825055
A_52_P265820 0.192665214
A_52_P27056 -0.14310854
A_52_P61833 0.301816018
A_51_P375631 0.642484253
A_51_P432011 -0.074479974
A_52_P160057 2.977107849
A_52_P633785 0.889216786
A_51_P204237 0.092008729
A_52_P1053160 -0.100714897
A_52_P677891 3.595481068
A_52_P352541 2.109196877

Total number of rows: 20674

Table truncated, full table size 510 Kbytes.




Supplementary file Size Download File type/resource
GSM2389465_Agilent_251486827983_S01_GE2_107_Sep09_1_3.txt.gz 14.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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