|
Status |
Public on Oct 03, 2017 |
Title |
CS6688 Replicate 2 |
Sample type |
RNA |
|
|
Source name |
Aerial parts of seedling
|
Organism |
Arabidopsis thaliana |
Characteristics |
accession: CS6688 age: 18-days-old tissue: Aerial parts of seedling
|
Growth protocol |
18-days-old seedlings were growing on 1% agar plates containing Murashige and Skoog (MS)
|
Extracted molecule |
total RNA |
Extraction protocol |
Approximately 500 μg total RNA was extracted from each sample using TRIzol (Invitrogen)
|
Label |
Cy3
|
Label protocol |
labeling were performed using the Agilent Low RNA Input Linear Amplification Kit (Agilent Technology), according to the manufacturer’s protocol.
|
|
|
Hybridization protocol |
Hybridization was performed using the in situ Hybridization Kit Plus (Agilent Technologies).
|
Scan protocol |
Scanned on an Agilent G2505 BDNA microarray scanner (Agilent Technologies)
|
Description |
Gene expression of CS6688
|
Data processing |
Data analysis was performed by Feature Extraction and Image Analysis software (Agilent Technologies)
|
|
|
Submission date |
Nov 14, 2016 |
Last update date |
Oct 03, 2017 |
Contact name |
Kazumasa Shirai |
E-mail(s) |
shirai.kazumasa348@mail.kyutech.jp
|
Phone |
0948-29-7842
|
Organization name |
Kyushu Institute of Technology
|
Department |
Frontier Research Academy for Young Researchers
|
Lab |
Hanada lab
|
Street address |
680-4 Kawazu
|
City |
Iizuka |
State/province |
Fukuoka |
ZIP/Postal code |
820-8502 |
Country |
Japan |
|
|
Platform ID |
GPL14937 |
Series (1) |
GSE89805 |
Transcriptome analysis of annotated coding genes and sORF at 74 accessions of A. thaliana |
|