|
| Status |
Public on Jan 28, 2008 |
| Title |
mnn-affy-5: heterozygote - X84_X46 female |
| Sample type |
RNA |
| |
|
| Source name |
heterozygote - X84_X46 female
|
| Organism |
Drosophila melanogaster |
| Characteristics |
w[67c];P{wHy}30G01DX84/P{wHy}30G01DX46 adult females
|
| Growth protocol |
Flies were grown at 25°C on PB medium (KD Medical, Columbia, MD) for 3 to 5 days post eclosion. Adults were then collected and quick frozen on dry ice and total RNA was extracted (Andrews et al, Genome Research 10:2030-2043) using Trizol reagent (GibcoBRL, Gaithersburg, MD).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Adults were collected and quick frozen on dry ice and total RNA was extracted (Andrews et al, Genome Research 10:2030-2043) using Trizol reagent (GibcoBRL, Gaithersburg, MD).
|
| Label |
Biotin
|
| Label protocol |
Labelling was performed by the NIDDK core facility and is described at http://genomics.niddk.nih.gov/
|
| |
|
| Hybridization protocol |
Hybridization was performed by the NIDDK core facility and is described at http://genomics.niddk.nih.gov/
|
| Scan protocol |
Scanning was performed by the NIDDK core facility and is described at http://genomics.niddk.nih.gov/
|
| Description |
Sample is from flies homozygous for protein-null Mnn1 alleles and is described in detail in Cerrato et al., Dev Biol. 2006 Oct 1;298(1):59-70.
|
| Data processing |
CEL files were processed using the Bioconductor Affy package, version 2.12.0 (Bolstad et al., 2003; Irizarry et al., 2003). The Robust Multichip Average (RMA) function was used to normalize intensities and extract expression value. Specifically, RMA implements a probe specific correction of the PM probes using a model based on observed intensity (the sum of signal and noise), followed by normalization of corrected PM probes using quantile normalization, and finally, calculation of expression measure using a median polish.
|
| |
|
| Submission date |
Oct 24, 2007 |
| Last update date |
Apr 25, 2012 |
| Contact name |
Brian Oliver |
| E-mail(s) |
briano@nih.gov
|
| Phone |
301-204-9463
|
| Organization name |
NIDDK, NIH
|
| Department |
LBG
|
| Lab |
Developmental Genomics
|
| Street address |
50 South Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE9425 |
Expression profile analysis of menin1 mutants |
|
