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Sample GSM239578 Query DataSets for GSM239578
Status Public on Jan 28, 2008
Title mnn-affy-11: mnn- Y79_Y79 male
Sample type RNA
 
Source name mnn- Y79_Y79 male
Organism Drosophila melanogaster
Characteristics w[67c];P{wHy}30G01DY79/P{wHy}30G01DY79 adult females
Growth protocol Flies were grown at 25°C on PB medium (KD Medical, Columbia, MD) for 3 to 5 days post eclosion. Adults were then collected and quick frozen on dry ice and total RNA was extracted (Andrews et al, Genome Research 10:2030-2043) using Trizol reagent (GibcoBRL, Gaithersburg, MD).
Extracted molecule total RNA
Extraction protocol Adults were collected and quick frozen on dry ice and total RNA was extracted (Andrews et al, Genome Research 10:2030-2043) using Trizol reagent (GibcoBRL, Gaithersburg, MD).
Label Biotin
Label protocol Labelling was performed by the NIDDK core facility and is described at http://genomics.niddk.nih.gov/
 
Hybridization protocol Hybridization was performed by the NIDDK core facility and is described at http://genomics.niddk.nih.gov/
Scan protocol Scanning was performed by the NIDDK core facility and is described at http://genomics.niddk.nih.gov/
Description Sample is from flies homozygous for protein-null Mnn1 alleles and is described in detail in Cerrato et al., Dev Biol. 2006 Oct 1;298(1):59-70.
Data processing CEL files were processed using the Bioconductor Affy package, version 2.12.0 (Bolstad et al., 2003; Irizarry et al., 2003). The Robust Multichip Average (RMA) function was used to normalize intensities and extract expression value. Specifically, RMA implements a probe specific correction of the PM probes using a model based on observed intensity (the sum of signal and noise), followed by normalization of corrected PM probes using quantile normalization, and finally, calculation of expression measure using a median polish.
 
Submission date Oct 24, 2007
Last update date Apr 25, 2012
Contact name Brian Oliver
E-mail(s) briano@nih.gov
Phone 301-204-9463
Organization name NIDDK, NIH
Department LBG
Lab Developmental Genomics
Street address 50 South Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL72
Series (1)
GSE9425 Expression profile analysis of menin1 mutants

Data table header descriptions
ID_REF ID to link data back to GPL72 platform
VALUE RMA normalized intensities

Data table
ID_REF VALUE
141200_at 3.7836
141201_at 5.07668
141202_at 5.86702
141203_at 6.17319
141204_at 6.45364
141205_at 5.3388
141206_at 6.62869
141207_at 3.82353
141208_at 8.43431
141209_at 6.65762
141210_at 4.40617
141211_at 5.44026
141212_at 9.16449
141213_at 2.97619
141214_at 7.78939
141215_at 6.77187
141216_at 6.73129
141217_at 7.17095
141218_at 6.66288
141219_at 3.85408

Total number of rows: 14010

Table truncated, full table size 246 Kbytes.




Supplementary file Size Download File type/resource
GSM239578.CEL.gz 2.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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