|
| Status |
Public on Jun 13, 2017 |
| Title |
B6/RD7 E18, Replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
B6 E18 Pooled Retina
|
| Organism |
Mus musculus |
| Characteristics |
tissue: retina
strain: C57BL/6
developmental stage: Embryonic Day 18
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated from retinas (0.5-250mg of tissue) using mirVana miRNA isolation system (Ambion)
|
| Label |
Cy5
|
| Label protocol |
500 ng of total RNA from each sample were labeled with either CY5 or CY3 using the miRCURY LNA Power Labeling kit from Exiqon.
|
| |
|
| Channel 2 |
| Source name |
RD7 E18 Pooled Retina
|
| Organism |
Mus musculus |
| Characteristics |
tissue: retina
strain: RD7
developmental stage: Embryonic Day 18
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated from retinas (0.5-250mg of tissue) using mirVana miRNA isolation system (Ambion)
|
| Label |
Cy3
|
| Label protocol |
500 ng of total RNA from each sample were labeled with either CY5 or CY3 using the miRCURY LNA Power Labeling kit from Exiqon.
|
| |
|
| |
| Hybridization protocol |
Following Cy5/Cy3 labeling, samples resuspended in 12.5 ul of H2O (provided by Exiqon). The two samples were then mixed together (25 ul total) and 25 ul of 2x hybridization buffer from Exiqon was added and hybridized overnight at 56 degrees at on a MAUI 4-bay hybridization station for 16 hours. Slides were then washed with Exicon wash buffers per manufacturers suggestion.
|
| Scan protocol |
Scanned on GenePix 4000B (Axon Instruments) and resultant .TIF files gridded by and analyzed by GenePix 6.3 to capture intensities
|
| Description |
Biological replicate 1 of 3
|
| Data processing |
Data collection by GenePix; output to GPR/TIF files; Normalized using Lowess normalization; analysis performed with BRB Array Tools (Excel, Bioconductor, and R).
Log Ratio (635/532) [Cy5/Cy3] in processed_data.txt supplementary file
miRBase v 11
|
| |
|
| Submission date |
Dec 08, 2016 |
| Last update date |
Jun 13, 2017 |
| Contact name |
Neena B Haider |
| E-mail(s) |
neena_haider@meei.harvard.edu
|
| Phone |
6179120270
|
| Organization name |
Schepens Eye Research Institute/MEE/Harvard Medical School
|
| Department |
Ophthalmology
|
| Street address |
20 Staniford Steret
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL16346 |
| Series (1) |
| GSE91029 |
Multimodal Regulation Orchestrates Normal and Complex Disease States in the Retina |
|
