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Sample GSM2420222 Query DataSets for GSM2420222
Status Public on Dec 09, 2016
Title MDA_231_IMPI
Sample type RNA
 
Source name MDA-231-IMPI
Organism Homo sapiens
Characteristics cell line: MDA231 (parental)
cell type: breast cancer cells
genotype/variation: IMP1-expression
Growth protocol Cells were cultured in cultured DMEM supplemented with 10% fetal bovine serum, 100U/ml penicillin, and 100 µg/ml streptomycin at 37 °C in a humid environment with 5 % CO2
Extracted molecule total RNA
Extraction protocol Total RNA from cultured cells was extracted using RNAsimple Total RNA Kit(Tiangen, Beijing,China) according to the manufacturer's instructions.
Label Cy3
Label protocol Total RNA was amplified and labeled by Low Input Quick Amp Labeling Kit, One‐Color (Cat#5190‐2305, Agilent technologies, Santa Clara, CA, US), following the manufacturer’s instructions. Cy3-Labeled cRNA were purified by RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany). Labeling was performed by the Shanghai Biotechnology Corporation in Shanghai, China.
 
Hybridization protocol Each Slide was hybridized with 1.65μg Cy3‐labeled cRNA using Gene Expression Hybridization Kit (Cat#5188‐5242, Agilent technologies, Santa Clara, CA, US) in Hybridization Oven (Cat#G2545A, Agilent technologies, Santa Clara, CA, US), according to the manufacturer’s instructions. The experiment was performed by the ' Shanghai Biotechnology Corporation ' in Shanghai, China.
Scan protocol Slides were scanned by Agilent Microarray Scanner (Cat#G2565CA, Agilent technologies, Santa Clara, CA, US) with default settings, Dye channel: Green, Scan resolution=3μm, 20bit.
Description LncRNA expression profiles of IMP1-expression MDA231 cells
Data processing Data were extracted with Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US). Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US). The analysis was performed in the Shanghai Biotechnology Corporation in Shanghai, China.
 
Submission date Dec 08, 2016
Last update date Dec 09, 2016
Contact name Zhou Yanchun
E-mail(s) zyc2013st@foxmail.com
Organization name Shantou University Medical College
Department Pathophysiology
Street address Xinling Road
City Shantou
State/province Guangdong
ZIP/Postal code 515041
Country China
 
Platform ID GPL21242
Series (1)
GSE91057 Long Noncoding RNA Expression profiling of IMP1-expression and IMP1 nonexpression MDA231 Cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity
Flags

Data table
ID_REF VALUE Flags
LNCA_21_P0011174 1.5838735 A
CUST_8647_PI429285431 2.4966278 A
CUST_1486_PI429285431 3.3157368 A
CUST_1231_PI429285431 1.6040981 A
CUST_4510_PI429285431 2.7616436 A
CUST_28243_PI429285431 2.3001037 A
CUST_18082_PI429285431 16.84277 P
CUST_38235_PI429285431 13.218542 P
CUST_50661_PI429285431 2.3240802 A
CUST_3396_PI429285431 1.5673828 A
CUST_39635_PI429285431 2.7252607 A
CUST_48426_PI429285431 2.7521865 A
CUST_32589_PI429285431 2.4198792 A
CUST_12810_PI429285431 5.824458 P
CUST_8341_PI429285431 4.931091 P
CUST_40507_PI429285431 1.8920939 A
CUST_40866_PI429285431 3.105391 A
CUST_23859_PI429285431 2.495844 A
CUST_15264_PI429285431 1.5724669 A
CUST_40519_PI429285431 5.848927 P

Total number of rows: 63431

Table truncated, full table size 2072 Kbytes.




Supplementary file Size Download File type/resource
GSM2420222_MDA_231_IMPI_256022810133_S01_GE1_107_Sep09_1_3.txt.gz 25.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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