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Sample GSM242222 Query DataSets for GSM242222
Status Public on May 08, 2008
Title US-1078955 vs. Human Liver 71
Sample type RNA
 
Channel 1
Source name Liver
Organism Homo sapiens
Characteristics US-1078955
Treatment protocol No treatment
Extracted molecule total RNA
Extraction protocol Trizol Lysis + RNeasy Column
Label Cy3
Label protocol One round of RT-IVT to cRNA
 
Channel 2
Source name Liver
Organism Homo sapiens
Characteristics pool
Treatment protocol No treatment
Extracted molecule total RNA
Extraction protocol Trizol Lysis + RNeasy Column
Label Cy5
Label protocol One round of RT-IVT to cRNA
 
 
Hybridization protocol Labeled cRNAs were fragmented to an average size of approximately 50 to 100 nucleotides in the presence of 10 mM zinc chloride and adding a hybridization buffer containing 1 M sodium chloride, 0.5 percent sodium sarcosine, 50 mM morpholino-ethane sulfonic acid (pH 6.5), and formamide (final concentration, 30 percent at 40°C); After hybridization, the slides were washed
Scan protocol slides were scanned using a confocal laser scanner (Agilent Technologies, Palo Alto, CA). Fluorescence intensities of the scanned images were quantified, normalized, and balanced
Description Liver samples (1-2 g) were acquired from Caucasian individuals from three independent liver collections at tissue resource centers at Vanderbilt University, University of Pittsburg, and Merck Research Laboratories. All individuals were compared to a common pool created from equal portions of RNA from 191 (111 from Vanderbilt University and 80 from University of Pittsburg) samples.
Data processing Rosetta Resolver Ratio Experiment using Rosetta Resolver Agilent Error Model
 
Submission date Nov 12, 2007
Last update date May 08, 2008
Contact name eric e schadt
E-mail(s) eric_schadt@merck.com
Organization name Merck
Department Genetics
Street address 401 Terry Ave N
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL4372
Series (1)
GSE9588 Mapping the Genetic Architecture of Gene Expression in Human Liver

Data table header descriptions
ID_REF Rosetta generated unique probe identifier
VALUE same as UNF_VALUE but with flagged values removed
LOGINTENSITY Corrected average log intensity of channels
PVALUE P-value of LogRatio
QUALITY 1 - if good and non control, 0 - otherwise
UNF_VALUE Corrected Log10 Ratio of channels (CH2/CH1)

Data table
ID_REF VALUE LOGINTENSITY PVALUE QUALITY UNF_VALUE
10019475365 -0.0936 -0.5138 4.7206e-001 1 -0.0936
10019481149 -0.3693 -0.1981 9.1059e-003 1 -0.3693
10019495284 1.0940 -0.5728 5.2976e-006 1 1.0940
10019687586 0.1908 -1.3957 3.9853e-001 1 0.1908
10019713746 -0.1295 -0.3443 3.1205e-001 1 -0.1295
10019799479 -0.1345 -0.4913 3.0381e-001 1 -0.1345
10019809115 -0.0025 -0.8437 9.8600e-001 1 -0.0025
10019874890 -0.0397 -0.8729 7.8720e-001 1 -0.0397
10019903058 0.3733 -1.7693 3.3136e-001 1 0.3733
10019909307 -0.0365 -0.6861 7.8703e-001 1 -0.0365
10019911222 0.1855 -0.4270 1.5772e-001 1 0.1855
10019924807 -0.2487 -1.5127 3.9079e-001 1 -0.2487
10019927856 0.4349 -0.2917 3.3403e-003 1 0.4349
10019932383 -0.0860 -0.4786 5.0560e-001 1 -0.0860
10019948931 0.0433 -1.2399 8.2444e-001 1 0.0433
10019975533 0.1392 -1.1441 4.3083e-001 1 0.1392
10019977224 0.4126 -0.5202 5.5250e-003 1 0.4126
10019977227 0.0718 -0.1124 5.6456e-001 1 0.0718
10019987588 -0.0085 -0.8809 9.5407e-001 1 -0.0085
10020008603 -0.1981 -0.3943 1.3254e-001 1 -0.1981

Total number of rows: 39302

Table truncated, full table size 1873 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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