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Status |
Public on Dec 15, 2016 |
Title |
195 T |
Sample type |
RNA |
|
|
Source name |
esophageal tumor
|
Organism |
Homo sapiens |
Characteristics |
tissue: esophageal tumor
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy Tissue kit (Qiagen, Germantown, MD).
|
Label |
biotin
|
Label protocol |
Five mg of total RNA was reverse-transcribed using oligo-dT primers (Invitrogen) and SuperScript II reverse transcriptase (Invitrogen) in the presence of 100 mCi of [33P]dCTP (Amersham Bioscience, Piscataway, NJ) according to the instructions for the Gene
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Hybridization protocol |
The GeneFilters were prehybridized for 2 h at 51C with 0.5 mg/ml of poly-dA (Invitrogen) and 0.5 mg/ml Cot-1 DNA (Invitrogen) in 10 ml of AlkPhos DIRECT hybridization buffer (Amersham Bioscience) and then hybridized for 17 h at 51C with the denatured radi
|
Scan protocol |
The arrays were then exposed for 2 hours to an Imaging Plate and scanned at 25-mm resolution with a BAS5000 phosphoimager (Fuji Photo Film), images of the hybridized arrays were processed with L Process (Fuji Photo Film) and signal intensities quantified
|
Description |
1012WE0025
|
Data processing |
Data was quality controled and processed using Affymetrix Gene Expression Console.
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|
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Submission date |
Dec 14, 2016 |
Last update date |
Dec 15, 2016 |
Contact name |
Yan Guo |
E-mail(s) |
yanguo1978@gmail.com
|
Organization name |
Vanderbilt Cancer Center
|
Street address |
639 Preston Research Bld
|
City |
Nashville |
State/province |
TN |
ZIP/Postal code |
37232 |
Country |
USA |
|
|
Platform ID |
GPL6244 |
Series (1) |
GSE92396 |
Esophageal adenocarcinoma gene expression profiling |
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