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Sample GSM2428978 Query DataSets for GSM2428978
Status Public on Dec 15, 2016
Title 195 T
Sample type RNA
 
Source name esophageal tumor
Organism Homo sapiens
Characteristics tissue: esophageal tumor
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy Tissue kit (Qiagen, Germantown, MD).
Label biotin
Label protocol Five mg of total RNA was reverse-transcribed using oligo-dT primers (Invitrogen) and SuperScript II reverse transcriptase (Invitrogen) in the presence of 100 mCi of [33P]dCTP (Amersham Bioscience, Piscataway, NJ) according to the instructions for the Gene
 
Hybridization protocol The GeneFilters were prehybridized for 2 h at 51C with 0.5 mg/ml of poly-dA (Invitrogen) and 0.5 mg/ml Cot-1 DNA (Invitrogen) in 10 ml of AlkPhos DIRECT hybridization buffer (Amersham Bioscience) and then hybridized for 17 h at 51C with the denatured radi
Scan protocol The arrays were then exposed for 2 hours to an Imaging Plate and scanned at 25-mm resolution with a BAS5000 phosphoimager (Fuji Photo Film), images of the hybridized arrays were processed with L Process (Fuji Photo Film) and signal intensities quantified
Description 1012WE0025
Data processing Data was quality controled and processed using Affymetrix Gene Expression Console.
 
Submission date Dec 14, 2016
Last update date Dec 15, 2016
Contact name Yan Guo
E-mail(s) yanguo1978@gmail.com
Organization name Vanderbilt Cancer Center
Street address 639 Preston Research Bld
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
 
Platform ID GPL6244
Series (1)
GSE92396 Esophageal adenocarcinoma gene expression profiling

Data table header descriptions
ID_REF
VALUE RMA normalized gene expression value

Data table
ID_REF VALUE
8104463 10.48312
7917455 10.10789
7892501 2.134113
7892502 3.983595
7892503 3.252141
7892504 9.018606
7892505 2.895487
7892506 3.750804
7892507 4.426469
7892508 6.151167
7892509 10.42688
7892510 4.394455
7892511 3.226269
7892512 6.955833
7892513 2.673453
7892514 10.99408
7892515 9.789059
7892516 4.019892
7892517 5.612702
7892518 2.765293

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM2428978_1012WE0025-20101014-HuGene.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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