|
| Status |
Public on Dec 15, 2016 |
| Title |
44 N |
| Sample type |
RNA |
| |
|
| Source name |
normal esophageal tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: normal esophageal tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Tissue kit (Qiagen, Germantown, MD).
|
| Label |
biotin
|
| Label protocol |
Five mg of total RNA was reverse-transcribed using oligo-dT primers (Invitrogen) and SuperScript II reverse transcriptase (Invitrogen) in the presence of 100 mCi of [33P]dCTP (Amersham Bioscience, Piscataway, NJ) according to the instructions for the Gene
|
| |
|
| Hybridization protocol |
The GeneFilters were prehybridized for 2 h at 51C with 0.5 mg/ml of poly-dA (Invitrogen) and 0.5 mg/ml Cot-1 DNA (Invitrogen) in 10 ml of AlkPhos DIRECT hybridization buffer (Amersham Bioscience) and then hybridized for 17 h at 51C with the denatured radi
|
| Scan protocol |
The arrays were then exposed for 2 hours to an Imaging Plate and scanned at 25-mm resolution with a BAS5000 phosphoimager (Fuji Photo Film), images of the hybridized arrays were processed with L Process (Fuji Photo Film) and signal intensities quantified
|
| Description |
1012WE0028
|
| Data processing |
Data was quality controled and processed using Affymetrix Gene Expression Console.
|
| |
|
| Submission date |
Dec 14, 2016 |
| Last update date |
Dec 15, 2016 |
| Contact name |
Yan Guo |
| E-mail(s) |
yanguo1978@gmail.com
|
| Organization name |
Vanderbilt Cancer Center
|
| Street address |
639 Preston Research Bld
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE92396 |
Esophageal adenocarcinoma gene expression profiling |
|
