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Sample GSM2433555 Query DataSets for GSM2433555
Status Public on Dec 19, 2019
Title PTEN KO sample num 336
Sample type SRA
Source name Region I epididymis tissue
Organism Mus musculus
Characteristics condition: PTEN knockout
tissue: Region I epididymis
Treatment protocol Mice carrying loxP-flanked Pten allele (Pten flox/flox) were purchased from The Jackson Laboratory (Bar Harbor, Maine). Tg(Rnase10-Cre) mice were a generous gift from Dr. Huhtaniemi (Imperial College London, UK) (Krutskikh et al., 2012). Mice were bred and genotyped based upon the information provided by The Jackson Laboratory (Bar Harbor, Maine).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Qiagen RNeasy Plus Mini Kit (Valencia, CA) according to manufacturer's instructions.
Total RNA was extracted and an mRNA library was prepared using Illumina TruSeq platform and followed by transcriptome sequencing using an Illumina HiSeq 2000.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Description P336_KO_TTAGGC
Data processing Alignment: STAR 2.4.0
Quantitation: featureCounts 1.4.4
Normalization & Differential Expression: DESeq2 1.4.5
Genome_build: GRCm38
Supplementary_files_format_and_content: xu-counts.csv: contains raw gene-level counts against GRCm38 GENCODE genes
Submission date Dec 20, 2016
Last update date Dec 19, 2019
Contact name Stephen Turner
Organization name Signature Science, LLC
Street address 1670 Discovery Drive
City Charlottesville
State/province VA
ZIP/Postal code 22911
Country USA
Platform ID GPL13112
Series (1)
GSE92614 PTEN is essential in the maintenance of normal transporter activity in the epididymal epithelium
BioSample SAMN06167058
SRA SRX2437063

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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