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Sample GSM243420 Query DataSets for GSM243420
Status Public on Aug 26, 2008
Title Control, GDX+vehicle; female, replicate
Sample type RNA
 
Source name adipose tissue; Control, GDX+vehicle
Organism Mus musculus
Characteristics Female mice retroperitoneal adipose tissue
Treatment protocol DHT (0.1mg) was injected subcutaneously 1, 3, 6, 12, 18 and 24 h prior to sacrifice in DHT-treated groups. One group was retained as control and received a vehicle solution.
Extracted molecule total RNA
Extraction protocol Retroperitoneal adipose tissue samples from all mice of the same group were collected, rapidly trimmed, snap-frozen in liquid nitrogen and stored at -80ºC prior to mRNA extraction.
Total RNA was isolated from cells using Trizol Reagent, according to the manufacturer’s protocol (Invitrogen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C to GeneChip MG-U74A v2 Genechip Set. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using an Agilent GeneArray Scanncer (Agilent Technologies).
Description Gene expression data from adipose tissue, 24h prior to necropsy.
Data processing CEL files were extracted with Microarray Suite 5.0 (Affymetrix).
 
Submission date Nov 16, 2007
Last update date Aug 14, 2011
Contact name Ezequiel L Calvo
E-mail(s) cezequiel@yahoo.com
Organization name CRCHUL
Department Molecular Endocrinilogy
Lab Microarrays
Street address 2705 Boul. Laurier
City Quebec
State/province Quebec
ZIP/Postal code G1V 4G2
Country Canada
 
Platform ID GPL81
Series (1)
GSE9631 Response of the adipose tissue transcriptome to dihydrotestosterone in mice

Data table header descriptions
ID_REF
VALUE Signal intensity was calculated by AffylmGUI (Bioconductor).

Data table
ID_REF VALUE
100001_at 13.13878707
100002_at 25.61461086
100003_at 460.5934889
100004_at 293.9034939
100005_at 663.5172086
100006_at 89.6982515
100007_at 681.0560793
100009_r_at 37.3840325
100010_at 231.8543563
100011_at 201.753367
100012_at 3203.393948
100013_at 577.3458216
100014_at 104.2054531
100015_at 329.5431241
100016_at 121.422879
100017_at 198.6749876
100018_at 149.5740802
100019_at 725.7609749
100020_at 798.178956
100021_at 9.693509774

Total number of rows: 12488

Table truncated, full table size 267 Kbytes.




Supplementary file Size Download File type/resource
GSM243420.CEL.gz 2.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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