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Status |
Public on Aug 26, 2008 |
Title |
6h, GDX+DHT; female |
Sample type |
RNA |
|
|
Source name |
adipose tissue; 6h, GDX+DHT
|
Organism |
Mus musculus |
Characteristics |
Female mice retroperitoneal adipose tissue
|
Treatment protocol |
DHT (0.1mg) was injected subcutaneously 1, 3, 6, 12, 18 and 24 h prior to sacrifice in DHT-treated groups. One group was retained as control and received a vehicle solution.
|
Extracted molecule |
total RNA |
Extraction protocol |
Retroperitoneal adipose tissue samples from all mice of the same group were collected, rapidly trimmed, snap-frozen in liquid nitrogen and stored at -80ºC prior to mRNA extraction. Total RNA was isolated from cells using Trizol Reagent, according to the manufacturer’s protocol (Invitrogen).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C to GeneChip MG-U74A v2 Genechip Set. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using an Agilent GeneArray Scanncer (Agilent Technologies).
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Description |
Gene expression data from adipose tissue, 6h prior to necropsy.
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Data processing |
CEL files were extracted with Microarray Suite 5.0 (Affymetrix).
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|
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Submission date |
Nov 16, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Ezequiel L Calvo |
E-mail(s) |
cezequiel@yahoo.com
|
Organization name |
CRCHUL
|
Department |
Molecular Endocrinilogy
|
Lab |
Microarrays
|
Street address |
2705 Boul. Laurier
|
City |
Quebec |
State/province |
Quebec |
ZIP/Postal code |
G1V 4G2 |
Country |
Canada |
|
|
Platform ID |
GPL81 |
Series (1) |
GSE9631 |
Response of the adipose tissue transcriptome to dihydrotestosterone in mice |
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