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Sample GSM243423 Query DataSets for GSM243423
Status Public on Aug 26, 2008
Title 6h, GDX+DHT; female
Sample type RNA
 
Source name adipose tissue; 6h, GDX+DHT
Organism Mus musculus
Characteristics Female mice retroperitoneal adipose tissue
Treatment protocol DHT (0.1mg) was injected subcutaneously 1, 3, 6, 12, 18 and 24 h prior to sacrifice in DHT-treated groups. One group was retained as control and received a vehicle solution.
Extracted molecule total RNA
Extraction protocol Retroperitoneal adipose tissue samples from all mice of the same group were collected, rapidly trimmed, snap-frozen in liquid nitrogen and stored at -80ºC prior to mRNA extraction.
Total RNA was isolated from cells using Trizol Reagent, according to the manufacturer’s protocol (Invitrogen).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C to GeneChip MG-U74A v2 Genechip Set. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using an Agilent GeneArray Scanncer (Agilent Technologies).
Description Gene expression data from adipose tissue, 6h prior to necropsy.
Data processing CEL files were extracted with Microarray Suite 5.0 (Affymetrix).
 
Submission date Nov 16, 2007
Last update date Aug 14, 2011
Contact name Ezequiel L Calvo
E-mail(s) cezequiel@yahoo.com
Organization name CRCHUL
Department Molecular Endocrinilogy
Lab Microarrays
Street address 2705 Boul. Laurier
City Quebec
State/province Quebec
ZIP/Postal code G1V 4G2
Country Canada
 
Platform ID GPL81
Series (1)
GSE9631 Response of the adipose tissue transcriptome to dihydrotestosterone in mice

Data table header descriptions
ID_REF
VALUE Signal intensity was calculated by AffylmGUI (Bioconductor).

Data table
ID_REF VALUE
100001_at 25.62881291
100002_at 18.02104759
100003_at 727.7032638
100004_at 285.3023039
100005_at 583.9932943
100006_at 95.79867952
100007_at 689.5728223
100009_r_at 34.8321309
100010_at 222.369759
100011_at 246.541908
100012_at 2384.139219
100013_at 501.5972543
100014_at 127.2741409
100015_at 238.1841178
100016_at 209.2754555
100017_at 201.2184293
100018_at 34.5295733
100019_at 650.5978113
100020_at 903.3489162
100021_at 22.88099207

Total number of rows: 12488

Table truncated, full table size 267 Kbytes.




Supplementary file Size Download File type/resource
GSM243423.CEL.gz 2.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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