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Status |
Public on Jan 31, 2018 |
Title |
35S::SUP-GR DEX_rep2 |
Sample type |
RNA |
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Source name |
35S::SUP-GR in Ler wild-type
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Organism |
Arabidopsis thaliana |
Characteristics |
genotype: 35S::SUP-GR in Ler wild-type treatment: DEX phenotype: DEX treatment will lead to reduced size in organs tissue: inflorescences with flower buds earlier than stage 10
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Treatment protocol |
10 µm dexamethasone diluted from a 30 mM stock in ethanol were used for the DEX treatment, while the Mock treatment was conducted with the same amount of ethanol, by inverting the plants and submerging the inflorescence for 1 min in solution containing either 10 µm DEX or ethanol, together with 0.015% Silwet L-77.
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Growth protocol |
The seeds of the indicated genotype were planted on soil, and grown at 22°C under continuous light conditions. The inflorescence with flower buds earlier than stage 10 was sampled from Mock and DEX-treated plants.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and the RNAprotect reagent (Qiagen) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen).
|
Label |
Cy3
|
Label protocol |
Labeling was performed following NimbleGen standard operating protocol. See www.nimblegen.com.
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Hybridization protocol |
Hybridization was performed following NimbleGen standard operating protocol. See www.nimblegen.com.
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Scan protocol |
Scanning was performed following NimbleGen standard operating protocol. See www.nimblegen.com.
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Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
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Submission date |
Dec 22, 2016 |
Last update date |
Jan 31, 2018 |
Contact name |
Yifeng XU |
E-mail(s) |
yifeng@tll.org.sg
|
Phone |
68727742
|
Organization name |
Temasek Life Sciences Laboratory
|
Street address |
1 Research Link
|
City |
Singapore |
State/province |
- |
ZIP/Postal code |
117604 |
Country |
Singapore |
|
|
Platform ID |
GPL13970 |
Series (1) |
GSE92729 |
Gene expression analysis of the inflorescence of 35S::SUP-GR after Mock and DEX treatment |
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