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Sample GSM2440441 Query DataSets for GSM2440441
Status Public on Jan 06, 2017
Title LPHN2-sh cells that underwent cardiac differentiation at day 3
Sample type protein
 
Source name cardiac differentiated cell from LPHN2-sh PSC
Organism Mus musculus
Characteristics genotype/variation: LPHN2-sh
cell type: cardiac differentiated cell
time: day 3
Extracted molecule protein
Extraction protocol 10~40mg of tissues were washed with cold 1X PBS. And tissue samples were homogenized with Extracting buffer (Fullmoon Bio). Debris was removed from tissue extract with centrifuge.
Label biotin
Label protocol 50ug of Tissue extract was labeled with Biotin and label buffer (Fullmoon Bio).
 
Hybridization protocol Biotin labeled extract was hybridized with antibody-spotted slide for 2hr at room temperature. After hybridization, slide was washed with 1X wash buffer (Fullmoon Bio) and de-ionized water. Detection Buffer including Cy3-Streptavidin (fullmoon Bio) was treated for 20min at room temperature. And slide was washed with 1X wash buffer (Fullmoon Bio) and de-ionized water.
Scan protocol Scanned on an Axon 4000B scanner.
Description LPHN2-Sh (3d)
Data processing GenePix Software (v 6.1) was used for data extraction and Genowiz software (v 4.0.5.6) was used for global normalization.
 
Submission date Dec 27, 2016
Last update date Jan 06, 2017
Contact name Choon-Soo Lee
E-mail(s) younkouni@gmail.com
Phone 82-10-7323-1391
Organization name Seoul National University Hospital
Street address 28 Yongon-Dong, Jongno-gu
City Seoul
ZIP/Postal code 110-744
Country South Korea
 
Platform ID GPL18873
Series (1)
GSE92923 Adhesion GPCR, latrophilin-2, specifies cardiac lineage commitment through CDK5, Src, and P38MAPK

Data table header descriptions
ID_REF
VALUE Global normalized signal intensity

Data table
ID_REF VALUE
1_4_1 9.77747
1_5_1 9.6599045
1_6_1 9.77747
1_7_1 10.27712
1_8_1 10.267323
1_9_1 9.414978
1_10_1 9.307211
1_11_1 9.268023
1_12_1 9.953817
1_13_1 9.130863
1_14_1 9.405182
1_15_1 8.915329
1_16_1 9.875441
1_17_1 9.434573
1_18_1 9.934223
1_19_1 9.914629
1_1_2 10.228134
1_2_2 9.895035
1_3_2 9.44437
1_4_2 10.17915

Total number of rows: 1323

Table truncated, full table size 20 Kbytes.




Supplementary file Size Download File type/resource
GSM2440441_LPHN2-Sh_3d_.gpr.gz 102.9 Kb (ftp)(http) GPR
Processed data included within Sample table

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