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Status |
Public on Jan 06, 2017 |
Title |
LPHN2-sh cells that underwent cardiac differentiation at day 4 |
Sample type |
protein |
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Source name |
cardiac differentiated cell from LPHN2-sh PSC
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Organism |
Mus musculus |
Characteristics |
genotype/variation: LPHN2-sh cell type: cardiac differentiated cell time: day 4
|
Extracted molecule |
protein |
Extraction protocol |
10~40mg of tissues were washed with cold 1X PBS. And tissue samples were homogenized with Extracting buffer (Fullmoon Bio). Debris was removed from tissue extract with centrifuge.
|
Label |
biotin
|
Label protocol |
50ug of Tissue extract was labeled with Biotin and label buffer (Fullmoon Bio).
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Hybridization protocol |
Biotin labeled extract was hybridized with antibody-spotted slide for 2hr at room temperature. After hybridization, slide was washed with 1X wash buffer (Fullmoon Bio) and de-ionized water. Detection Buffer including Cy3-Streptavidin (fullmoon Bio) was treated for 20min at room temperature. And slide was washed with 1X wash buffer (Fullmoon Bio) and de-ionized water.
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Scan protocol |
Scanned on an Axon 4000B scanner.
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Description |
LPHN2-Sh (4d)
|
Data processing |
GenePix Software (v 6.1) was used for data extraction and Genowiz software (v 4.0.5.6) was used for global normalization.
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Submission date |
Dec 27, 2016 |
Last update date |
Jan 06, 2017 |
Contact name |
Choon-Soo Lee |
E-mail(s) |
younkouni@gmail.com
|
Phone |
82-10-7323-1391
|
Organization name |
Seoul National University Hospital
|
Street address |
28 Yongon-Dong, Jongno-gu
|
City |
Seoul |
ZIP/Postal code |
110-744 |
Country |
South Korea |
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|
Platform ID |
GPL18873 |
Series (1) |
GSE92923 |
Adhesion GPCR, latrophilin-2, specifies cardiac lineage commitment through CDK5, Src, and P38MAPK |
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