GEO help: Mouse over screen elements for information.
Status
Public on Sep 19, 2002
Title
B7hi mTEC B6/129 wt1
Sample type
RNA
Source name
murine medullary thymic epithelial cells
Organism
Mus musculus
Extracted molecule
total RNA
Description
Mice used were wild type B6/129 F2's of 3-5 weeks of age. Thymi from five of these mice of both sexes were removed and pooled. After collagenase/dispase digestion, density gradient fractionation, and fluorescent antibody staining, cells with the phenotype CD45-, G8.8+, CDR1int and B7.1hi were FACS-sorted and total RNA was made from them. RNA was twice-amplified using a T7 polymerase-based method.
Submission date
Sep 18, 2002
Last update date
May 27, 2005
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu
Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL81
Series (1)
GSE85
wild type and aire -/- murine meduallary thymic epithelial cells
Data table header descriptions
ID_REF
VALUE
Microarray Suite 5.0 raw signal
ABS_CALL
Microarray Suite 5.0 call -- absent, present, marginal
NORM_VALUE
signal values after processing with "Norm" software
Data table
ID_REF
VALUE
ABS_CALL
NORM_VALUE
100001_at
5.7
A
5.15
100002_at
13.7
A
12.76
100003_at
4
A
3.58
100004_at
10.3
A
9.63
100005_at
9.7
A
9.14
100006_at
7.2
P
6.61
100007_at
14.2
A
13.33
100009_r_at
24.8
P
23.69
100010_at
1.6
A
1.36
100011_at
22.7
P
21.94
100012_at
201.7
P
203.58
100013_at
48.2
P
47.52
100014_at
9.7
P
9.06
100015_at
14.7
P
14.17
100016_at
5.9
A
5.45
100017_at
5.7
A
5.28
100018_at
1.5
A
1.41
100019_at
5.5
P
5.09
100020_at
14.9
A
14.44
100021_at
3.2
A
2.9
Total number of rows: 12488 Table truncated, full table size 260 Kbytes .Supplementary file
Size
Download
File type/resource
GSM2444.CEL.gz
2.5 Mb
(ftp) (http)
CEL