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Status
Public on Sep 19, 2002
Title
B7hi mTEC B6/129 aire-/- 1
Sample type
RNA
Source name
murine medullary thymic epithelial cells
Organism
Mus musculus
Extracted molecule
total RNA
Description
Mice used were B6/129 F2's, 3-5 weeks of age, with both copies of the autoimmune regulator gene (aire, GenBank #AF079536) disrupted. Thymi from five of these mice of both sexes were removed and pooled. After collagenase/dispase digestion, density gradient fractionation, and fluorescent antibody staining, cells with the phenotype CD45-, G8.8+, CDR1int and B7.1hi were FACS-sorted and total RNA was made from them. RNA was twice-amplified using a T7 polymerase-based method.
Submission date
Sep 18, 2002
Last update date
May 27, 2005
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu
Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL81
Series (1)
GSE85
wild type and aire -/- murine meduallary thymic epithelial cells
Data table header descriptions
ID_REF
VALUE
Microarray Suite 5.0 raw signal
ABS_CALL
Microarray Suite 5.0 call -- absent, present, marginal
NORM_VALUE
signal values after processing with "Norm" software
Data table
ID_REF
VALUE
ABS_CALL
NORM_VALUE
100001_at
4.8
A
4.82
100002_at
1.6
A
1.64
100003_at
0.9
A
1
100004_at
7.1
A
7.27
100005_at
10.2
A
10.48
100006_at
3.9
P
3.96
100007_at
2.2
A
2.26
100009_r_at
10.6
P
10.93
100010_at
1.9
A
1.83
100011_at
12.1
P
12.48
100012_at
136.5
P
132.66
100013_at
86.3
P
87.61
100014_at
5.1
P
5.22
100015_at
27.7
P
28.56
100016_at
6.6
A
6.72
100017_at
7.7
P
7.85
100018_at
4.2
A
4.31
100019_at
1.3
A
1.32
100020_at
31
P
31.96
100021_at
0.5
A
1.01
Total number of rows: 12488 Table truncated, full table size 260 Kbytes .Supplementary file
Size
Download
File type/resource
GSM2447.CEL.gz
2.5 Mb
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CEL