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Sample GSM2447 Query DataSets for GSM2447
Status Public on Sep 19, 2002
Title B7hi mTEC B6/129 aire-/- 1
Sample type RNA
 
Source name murine medullary thymic epithelial cells
Organism Mus musculus
Extracted molecule total RNA
 
Description Mice used were B6/129 F2's, 3-5 weeks of age, with both copies of the autoimmune regulator gene (aire, GenBank #AF079536) disrupted. Thymi from five of these mice of both sexes were removed and pooled. After collagenase/dispase digestion, density gradient fractionation, and fluorescent antibody staining, cells with the phenotype CD45-, G8.8+, CDR1int and B7.1hi were FACS-sorted and total RNA was made from them. RNA was twice-amplified using a T7 polymerase-based method.
 
Submission date Sep 18, 2002
Last update date May 27, 2005
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL81
Series (1)
GSE85 wild type and aire -/- murine meduallary thymic epithelial cells

Data table header descriptions
ID_REF
VALUE Microarray Suite 5.0 raw signal
ABS_CALL Microarray Suite 5.0 call -- absent, present, marginal
NORM_VALUE signal values after processing with "Norm" software

Data table
ID_REF VALUE ABS_CALL NORM_VALUE
100001_at 4.8 A 4.82
100002_at 1.6 A 1.64
100003_at 0.9 A 1
100004_at 7.1 A 7.27
100005_at 10.2 A 10.48
100006_at 3.9 P 3.96
100007_at 2.2 A 2.26
100009_r_at 10.6 P 10.93
100010_at 1.9 A 1.83
100011_at 12.1 P 12.48
100012_at 136.5 P 132.66
100013_at 86.3 P 87.61
100014_at 5.1 P 5.22
100015_at 27.7 P 28.56
100016_at 6.6 A 6.72
100017_at 7.7 P 7.85
100018_at 4.2 A 4.31
100019_at 1.3 A 1.32
100020_at 31 P 31.96
100021_at 0.5 A 1.01

Total number of rows: 12488

Table truncated, full table size 260 Kbytes.




Supplementary file Size Download File type/resource
GSM2447.CEL.gz 2.5 Mb (ftp)(http) CEL

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