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Sample GSM2451625 Query DataSets for GSM2451625
Status Public on Sep 05, 2018
Title sample5, dataset1
Sample type RNA
 
Source name breast tumor tissue
Organism Homo sapiens
Characteristics tissue: breast tumor
er status: negative
her2 status: positive
tissue preservation method: Fresh-Frozen
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Total RNA from frozen samples was extracted using TRIzol® Reagent according to Thermo Fisher Scientific's (Invitrogen) recommendations.
Label biotin
Label protocol Total RNA was amplified and labeled using Affymetrix's GeneChip® 3' IVT Express kit according to Affymetrix's recommendations.
 
Hybridization protocol On GeneChip® HG-U133 Plus2.0 array using GeneChip® Hybridization, Wash, and Stain Kit, according to Affymetrix's standard procedure.
Scan protocol Using GeneChip® scanner 3000 according to Affymetrix's standard procedure.
Data processing Data set was RMA background corrected,quantile normalized and median-polish summarized using 'expresso' function available from bioconductor package affy.
 
Submission date Jan 09, 2017
Last update date Sep 05, 2018
Contact name Vinu Jose
E-mail(s) vinu.jose@yahoo.co.in
Organization name Institut Jules Bordet – Université Libre de Bruxelles (ULB)
Lab Breast Cancer Translational Research Laboratory J.C. Heuson (ULB 290)
Street address Rue Meylemeersch 90
City Brussels
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL570
Series (2)
GSE93332 Fresh-Frozen samples profiled on HG-U133plus2 array with Affymetrix's GeneChip® 3' IVT Express kit
GSE93338 Feasibility of Developing Reliable Gene Expression Modules from FFPE Derived RNA Profiled on Affymetrix Arrays

Data table header descriptions
ID_REF
VALUE Normalized expression values in log2 scale

Data table
ID_REF VALUE
1007_s_at 10.3833946984076
1053_at 7.22470568059913
117_at 6.55673143551673
121_at 7.10889498520126
1255_g_at 3.2239396432305
1294_at 7.05250356960028
1316_at 7.29583068730271
1320_at 3.88166645588852
1405_i_at 7.91239962344047
1431_at 3.73519017542062
1438_at 6.48364510888803
1487_at 7.45830193537621
1494_f_at 4.49451800992923
1552256_a_at 7.34364212551085
1552257_a_at 8.83032747574373
1552258_at 5.67472813678307
1552261_at 4.12410219396643
1552263_at 6.11277076719529
1552264_a_at 6.81638814914097
1552266_at 2.75585721808964

Total number of rows: 54675

Table truncated, full table size 1479 Kbytes.




Supplementary file Size Download File type/resource
GSM2451625_set1_s5.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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