|
Status |
Public on Sep 05, 2018 |
Title |
sample5, dataset4 |
Sample type |
RNA |
|
|
Source name |
breast tumor tissue
|
Organism |
Homo sapiens |
Characteristics |
tissue: breast tumor er status: negative her2 status: positive tissue preservation method: FFPE
|
Treatment protocol |
NA
|
Growth protocol |
NA
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from FFPE sections was extracted using miRNeasy FFPE kit according to Qiagen recommendation.
|
Label |
biotin
|
Label protocol |
Total RNA was amplified and labeled using Nugen's Ovation® FFPE WTA System and EncoreTM Biotin Module respectively, according to Nugen's recommendation.
|
|
|
Hybridization protocol |
On Affymetrix® HG-U219 Array Plate using GeneTitan® System with GeneTitanTM Hybridization, Wash, and Stain Kit for 3'IVT Array, according to Affymetrix standard procedure.
|
Scan protocol |
Using GeneTitan System according to Affymetrix standard procedure.
|
Data processing |
Data set was RMA background corrected,quantile normalized and median-polish summarized using 'expresso' function available from bioconductor package affy.
|
|
|
Submission date |
Jan 09, 2017 |
Last update date |
Sep 05, 2018 |
Contact name |
Vinu Jose |
E-mail(s) |
vinu.jose@yahoo.co.in
|
Organization name |
Institut Jules Bordet – Université Libre de Bruxelles (ULB)
|
Lab |
Breast Cancer Translational Research Laboratory J.C. Heuson (ULB 290)
|
Street address |
Rue Meylemeersch 90
|
City |
Brussels |
ZIP/Postal code |
1070 |
Country |
Belgium |
|
|
Platform ID |
GPL13667 |
Series (2) |
GSE93336 |
FFPE samples profiled on HG-U219 array with Nugen's Ovation® FFPE WTA System and EncoreTM Biotin Module |
GSE93338 |
Feasibility of Developing Reliable Gene Expression Modules from FFPE Derived RNA Profiled on Affymetrix Arrays |
|