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Sample GSM2458515 Query DataSets for GSM2458515
Status Public on Jul 18, 2017
Title LNCaP_vehicle_rep1
Sample type RNA
Source name LNCaP cells, vehicle treatment
Organism Homo sapiens
Characteristics cell line: human LNCaP prostate cancer cells (immortalized cell line)
: vehicle
Treatment protocol Cells were treated for 48h with R1881 (10nM), Torin 1 (100nM), or vehicles (EtOH+DMSO).
Growth protocol Cells were seeded in RPMI media with no phenol red and with charcoal-stripped serum (no steroids) . 48h later, cell were treated with 10nM R1881, Torin 1 (100nM) or vehicles (EtOH and DMSO) for 48h. Triplicate samples were sent for each conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified using RNeasy mini kit from QIAGEN
Label biotin
Label protocol Labeling was performed using standard Illumina protocol at Génome Québec Innovation Centre (McGill University)
Hybridization protocol RNA was hybridized to Illumina HumanHT-12 Expression Beachip v4 and quantified at Génome Québec Innovation Centre (McGill University)
Scan protocol Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
Description vehicle1
Data processing The expression data were analyzed using FlexArray10 software.
FlexArray10 software was using for data normalization and expression comparison between groups.
Submission date Jan 13, 2017
Last update date Jul 18, 2017
Contact name Etienne Audet-Walsh
Organization name Université Laval
Department Molecular Medicine
Street address 2705 boulevard Laurier
City Québec City
State/province QC
ZIP/Postal code G1V 4G2
Country Canada
Platform ID GPL10558
Series (2)
GSE93603 Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression [array]
GSE93846 Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression

Data table header descriptions
VALUE normalized signal

Data table
ILMN_1728255 6.19476039133699
ILMN_1846128 6.18473090949136
ILMN_1651705 9.40592646772368
ILMN_1853167 6.32152223690222
ILMN_1695844 6.18446704039734
ILMN_1874323 6.33521763539878
ILMN_1666310 6.22667729696898
ILMN_1757152 6.27520697375061
ILMN_3236052 6.17852071475049
ILMN_2043569 6.38336545431518
ILMN_1811364 6.20441897236876
ILMN_1719498 6.56889843051802
ILMN_1763663 7.89060624239306
ILMN_1842320 6.19344608416066
ILMN_2278908 12.2712623876247
ILMN_1777359 6.19900648141348
ILMN_1779373 6.19750132536931
ILMN_1800412 6.50661991329323
ILMN_1812441 7.52738095130174
ILMN_1747946 6.20108868160559

Total number of rows: 47323

Table truncated, full table size 1381 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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