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Sample GSM2458516 Query DataSets for GSM2458516
Status Public on Jul 18, 2017
Title LNCaP_vehicle_rep2
Sample type RNA
Source name LNCaP cells, vehicle treatment
Organism Homo sapiens
Characteristics cell line: human LNCaP prostate cancer cells (immortalized cell line)
: vehicle
Treatment protocol Cells were treated for 48h with R1881 (10nM), Torin 1 (100nM), or vehicles (EtOH+DMSO).
Growth protocol Cells were seeded in RPMI media with no phenol red and with charcoal-stripped serum (no steroids) . 48h later, cell were treated with 10nM R1881, Torin 1 (100nM) or vehicles (EtOH and DMSO) for 48h. Triplicate samples were sent for each conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified using RNeasy mini kit from QIAGEN
Label biotin
Label protocol Labeling was performed using standard Illumina protocol at Génome Québec Innovation Centre (McGill University)
Hybridization protocol RNA was hybridized to Illumina HumanHT-12 Expression Beachip v4 and quantified at Génome Québec Innovation Centre (McGill University)
Scan protocol Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
Description vehicle2
Data processing The expression data were analyzed using FlexArray10 software.
FlexArray10 software was using for data normalization and expression comparison between groups.
Submission date Jan 13, 2017
Last update date Jul 18, 2017
Contact name Etienne Audet-Walsh
Organization name Université Laval
Department Molecular Medicine
Street address 2705 boulevard Laurier
City Québec City
State/province QC
ZIP/Postal code G1V 4G2
Country Canada
Platform ID GPL10558
Series (2)
GSE93603 Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression [array]
GSE93846 Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression

Data table header descriptions
VALUE normalized signal

Data table
ILMN_1728255 6.19547450510249
ILMN_1846128 6.19124113558879
ILMN_1651705 9.45921108147384
ILMN_1853167 6.19078991417944
ILMN_1695844 6.20803057411907
ILMN_1874323 6.48324344026169
ILMN_1666310 6.23448536802141
ILMN_1757152 6.22450664655933
ILMN_3236052 6.18516758980622
ILMN_2043569 6.37980641253375
ILMN_1811364 6.20614046507549
ILMN_1719498 6.57750198908622
ILMN_1763663 7.85754058858948
ILMN_1842320 6.18781293844136
ILMN_2278908 12.2329129065189
ILMN_1777359 6.1975418960387
ILMN_1779373 6.18896696637975
ILMN_1800412 6.61232306662524
ILMN_1812441 7.59268505680894
ILMN_1747946 6.17923241026019

Total number of rows: 47323

Table truncated, full table size 1381 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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