NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2458519 Query DataSets for GSM2458519
Status Public on Jul 18, 2017
Title LNCaP_vehicle+R1881_rep2
Sample type RNA
 
Source name LNCaP cells, 48h R1881 treatment
Organism Homo sapiens
Characteristics cell line: human LNCaP prostate cancer cells (immortalized cell line)
: R1881
Treatment protocol Cells were treated for 48h with R1881 (10nM), Torin 1 (100nM), or vehicles (EtOH+DMSO).
Growth protocol Cells were seeded in RPMI media with no phenol red and with charcoal-stripped serum (no steroids) . 48h later, cell were treated with 10nM R1881, Torin 1 (100nM) or vehicles (EtOH and DMSO) for 48h. Triplicate samples were sent for each conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified using RNeasy mini kit from QIAGEN
Label biotin
Label protocol Labeling was performed using standard Illumina protocol at Génome Québec Innovation Centre (McGill University)
 
Hybridization protocol RNA was hybridized to Illumina HumanHT-12 Expression Beachip v4 and quantified at Génome Québec Innovation Centre (McGill University)
Scan protocol Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
Description vehicler18812
Data processing The expression data were analyzed using FlexArray10 software.
FlexArray10 software was using for data normalization and expression comparison between groups.
 
Submission date Jan 13, 2017
Last update date Jul 18, 2017
Contact name Etienne Audet-Walsh
E-mail(s) etienne.audet-walsh@crchudequebec.ulaval.ca
Organization name Université Laval
Department Molecular Medicine
Street address 2705 boulevard Laurier
City Québec City
State/province QC
ZIP/Postal code G1V 4G2
Country Canada
 
Platform ID GPL10558
Series (2)
GSE93603 Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression [array]
GSE93846 Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
ILMN_1728255 6.17984392766175
ILMN_1846128 6.18913351516151
ILMN_1651705 8.82690876767431
ILMN_1853167 6.18771099082936
ILMN_1695844 6.19180840374701
ILMN_1874323 6.27910203365634
ILMN_1666310 6.22653386069074
ILMN_1757152 6.30229253391097
ILMN_3236052 6.18324765441116
ILMN_2043569 6.30423771426444
ILMN_1811364 6.19175886882564
ILMN_1719498 6.37309526913093
ILMN_1763663 7.84635235638432
ILMN_1842320 6.17940028647576
ILMN_2278908 12.6931218993585
ILMN_1777359 6.20734616179699
ILMN_1779373 6.20457518025367
ILMN_1800412 6.29294839832039
ILMN_1812441 7.16864370718098
ILMN_1747946 6.18824627702287

Total number of rows: 47323

Table truncated, full table size 1381 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap