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Sample GSM2458521 Query DataSets for GSM2458521
Status Public on Jul 18, 2017
Title LNCaP_vehicle+Torin 1_rep1
Sample type RNA
Source name LNCaP cells, 48h Torin 1 treatment
Organism Homo sapiens
Characteristics cell line: human LNCaP prostate cancer cells (immortalized cell line)
: Torin 1
Treatment protocol Cells were treated for 48h with R1881 (10nM), Torin 1 (100nM), or vehicles (EtOH+DMSO).
Growth protocol Cells were seeded in RPMI media with no phenol red and with charcoal-stripped serum (no steroids) . 48h later, cell were treated with 10nM R1881, Torin 1 (100nM) or vehicles (EtOH and DMSO) for 48h. Triplicate samples were sent for each conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified using RNeasy mini kit from QIAGEN
Label biotin
Label protocol Labeling was performed using standard Illumina protocol at Génome Québec Innovation Centre (McGill University)
Hybridization protocol RNA was hybridized to Illumina HumanHT-12 Expression Beachip v4 and quantified at Génome Québec Innovation Centre (McGill University)
Scan protocol Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
Description torinvehicle1
Data processing The expression data were analyzed using FlexArray10 software.
FlexArray10 software was using for data normalization and expression comparison between groups.
Submission date Jan 13, 2017
Last update date Jul 18, 2017
Contact name Etienne Audet-Walsh
Organization name Université Laval
Department Molecular Medicine
Street address 2705 boulevard Laurier
City Québec City
State/province QC
ZIP/Postal code G1V 4G2
Country Canada
Platform ID GPL10558
Series (2)
GSE93603 Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression [array]
GSE93846 Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression

Data table header descriptions
VALUE normalized signal

Data table
ILMN_1728255 6.21280461343099
ILMN_1846128 6.17527679230502
ILMN_1651705 9.399936596552
ILMN_1853167 6.23957487820103
ILMN_1695844 6.1966178992898
ILMN_1874323 6.2519540072645
ILMN_1666310 6.21440331003545
ILMN_1757152 6.20755402647338
ILMN_3236052 6.19437399003134
ILMN_2043569 6.33240009328
ILMN_1811364 6.20239171712221
ILMN_1719498 6.39139929389395
ILMN_1763663 7.74619437747148
ILMN_1842320 6.19557338719641
ILMN_2278908 12.2696713019629
ILMN_1777359 6.23119895623989
ILMN_1779373 6.20808756441706
ILMN_1800412 6.60166031300661
ILMN_1812441 7.55947931640073
ILMN_1747946 6.20753427429452

Total number of rows: 47323

Table truncated, full table size 1381 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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