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Sample GSM2458522 Query DataSets for GSM2458522
Status Public on Jul 18, 2017
Title LNCaP_vehicle+Torin 1_rep2
Sample type RNA
Source name LNCaP cells, 48h Torin 1 treatment
Organism Homo sapiens
Characteristics cell line: human LNCaP prostate cancer cells (immortalized cell line)
: Torin 1
Treatment protocol Cells were treated for 48h with R1881 (10nM), Torin 1 (100nM), or vehicles (EtOH+DMSO).
Growth protocol Cells were seeded in RPMI media with no phenol red and with charcoal-stripped serum (no steroids) . 48h later, cell were treated with 10nM R1881, Torin 1 (100nM) or vehicles (EtOH and DMSO) for 48h. Triplicate samples were sent for each conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified using RNeasy mini kit from QIAGEN
Label biotin
Label protocol Labeling was performed using standard Illumina protocol at Génome Québec Innovation Centre (McGill University)
Hybridization protocol RNA was hybridized to Illumina HumanHT-12 Expression Beachip v4 and quantified at Génome Québec Innovation Centre (McGill University)
Scan protocol Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
Description torinvehicle2
Data processing The expression data were analyzed using FlexArray10 software.
FlexArray10 software was using for data normalization and expression comparison between groups.
Submission date Jan 13, 2017
Last update date Jul 18, 2017
Contact name Etienne Audet-Walsh
Organization name Université Laval
Department Molecular Medicine
Street address 2705 boulevard Laurier
City Québec City
State/province QC
ZIP/Postal code G1V 4G2
Country Canada
Platform ID GPL10558
Series (2)
GSE93603 Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression [array]
GSE93846 Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression

Data table header descriptions
VALUE normalized signal

Data table
ILMN_1728255 6.19520700326494
ILMN_1846128 6.20161349967409
ILMN_1651705 8.90279121874088
ILMN_1853167 6.18964291773525
ILMN_1695844 6.19852046555458
ILMN_1874323 6.27952735447611
ILMN_1666310 6.19867664998164
ILMN_1757152 6.27657055109328
ILMN_3236052 6.18450759796261
ILMN_2043569 6.28943152711159
ILMN_1811364 6.23545778058536
ILMN_1719498 6.34874402066417
ILMN_1763663 7.6074339378292
ILMN_1842320 6.19616224840198
ILMN_2278908 12.5578347959373
ILMN_1777359 6.18330929770431
ILMN_1779373 6.18739889604456
ILMN_1800412 6.68588665850069
ILMN_1812441 7.49306080096606
ILMN_1747946 6.17240595973228

Total number of rows: 47323

Table truncated, full table size 1381 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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