|
| Status |
Public on Jul 18, 2017 |
| Title |
LNCaP_vehicle+Torin 1_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP cells, 48h Torin 1 treatment
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: human LNCaP prostate cancer cells (immortalized cell line)
: Torin 1
|
| Treatment protocol |
Cells were treated for 48h with R1881 (10nM), Torin 1 (100nM), or vehicles (EtOH+DMSO).
|
| Growth protocol |
Cells were seeded in RPMI media with no phenol red and with charcoal-stripped serum (no steroids) . 48h later, cell were treated with 10nM R1881, Torin 1 (100nM) or vehicles (EtOH and DMSO) for 48h. Triplicate samples were sent for each conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified using RNeasy mini kit from QIAGEN
|
| Label |
biotin
|
| Label protocol |
Labeling was performed using standard Illumina protocol at Génome Québec Innovation Centre (McGill University)
|
| |
|
| Hybridization protocol |
RNA was hybridized to Illumina HumanHT-12 Expression Beachip v4 and quantified at Génome Québec Innovation Centre (McGill University)
|
| Scan protocol |
Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
|
| Description |
torinvehicle3
|
| Data processing |
The expression data were analyzed using FlexArray10 software.
FlexArray10 software was using for data normalization and expression comparison between groups.
|
| |
|
| Submission date |
Jan 13, 2017 |
| Last update date |
Jul 18, 2017 |
| Contact name |
Etienne Audet-Walsh |
| E-mail(s) |
etienne.audet-walsh@crchudequebec.ulaval.ca
|
| Organization name |
Université Laval
|
| Department |
Molecular Medicine
|
| Street address |
2705 boulevard Laurier
|
| City |
Québec City |
| State/province |
QC |
| ZIP/Postal code |
G1V 4G2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE93603 |
Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression [array] |
| GSE93846 |
Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression |
|
