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Sample GSM2461445 Query DataSets for GSM2461445
Status Public on Apr 19, 2017
Title HeLa-siCTRL-T-rep1
Sample type SRA
Source name Human HeLa cells
Organism Homo sapiens
Characteristics cell line: HeLa
treatment: siCTRL
age: P7
tissue: carcinoma cervical
Extracted molecule total RNA
Extraction protocol RNA was isolated from siCTRL, siNSUN2 and ALYREF-RIP HeLa cells, and mutiple mouse tissues using the TRIzol (Invitrogen) reagent by following the company manual. For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100
Approximately 2.5 µg of total RNA was then used for library preparation using a TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol.The libraries were sequenced using HiSeq3000 (Illumina) or HiSeq2500 in paired-read mode, creating reads with a length of 101 or 125 bp
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
Description normal HeLa cells rep1
Data processing Reads were aligned to the hg19 or mm10 genome assembly using TopHat v2.0.9
For each sample, reads counts of all genes were computed using HTSeq v0.5.3p9
Genome_build: hg19 or mm10
Supplementary_files_format_and_content: all-samples-reads-count.xls
Submission date Jan 18, 2017
Last update date May 15, 2019
Contact name Bao-Fa Sun
Organization name Beijing Institute of Genomics (BIG) of Chinese Academy of Sciences (CAS)
Street address Da-Tun Road
City Beijing
ZIP/Postal code 100101
Country China
Platform ID GPL21290
Series (2)
GSE93750 Transcriptomics of siCTRL, siNSUN2 and ALYREF-RIP HeLa cells, and multiple mouse tissues
GSE93751 5-methylcytosine promotes mRNA export
BioSample SAMN06237543
SRA SRX2499547

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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