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Sample GSM2472789 Query DataSets for GSM2472789
Status Public on Nov 15, 2017
Title dex1
Sample type SRA
Source name inflorescence
Organism Arabidopsis thaliana
Characteristics ecotype: Columbia-0
tissue: inflorescence
age: 30 days after germination
developmental stage: until flower stage 3-4
Treatment protocol 0.01% ethanol,0.015% Silwet, with or without 10µM dexamethasone,
Growth protocol 23°C; 65% humidity; 16h light/8h dark; LED lights 200µE
Extracted molecule total RNA
Extraction protocol RNAeasy plant mini kit according to manufacturer instructions (Qiagen, Hilden, Germany)
The RNA were poly A selected using the NEB Poly (A) Magnetic isolation module (7490), then the NEBnext Ultra Direction RNA Prep Kit (E7420) for Illumina with the NEBnext Multiplex Oligo's for Illumina (E7335). For RNA 1 µg inout was used.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Data processing Quality control: FastQC
Read alignment: TOPHAT2
BAM SAM conversion: samtools
Read table reconstruction: HT-seq
Genome_build: TAIR10
Supplementary_files_format_and_content: text file with normalized read count table
Submission date Jan 31, 2017
Last update date May 15, 2019
Contact name Jan Lohmann
Phone +49186784234
Organization name Centre for Organismal Studies
Department Stem Cell Biology
Lab Jan Lohmann
Street address Im Neuenheimer Feld, 230
City Heidelberg
ZIP/Postal code 69120
Country Germany
Platform ID GPL19580
Series (2)
GSE94309 Control of plant cell fate transitions by transcriptional and hormonal signals [RNA-seq: mock_vs_dex_3h]
GSE94311 Control of plant cell fate transitions by transcriptional and hormonal signals
BioSample SAMN06284163
SRA SRX2529377

Supplementary file Size Download File type/resource
GSM2472789_ht_count_DEX1_cat.txt.gz 107.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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