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Sample GSM2494619 Query DataSets for GSM2494619
Status Public on Mar 13, 2018
Title Bovine macrophages stimulated with FhWey-ES, replicate 2
Sample type RNA
 
Channel 1
Source name BOMA, bovine macrophage cell line
Organism Bos taurus
Characteristics cell line: BOMA
cell type: monocytes/ macrophages
agent: none
Treatment protocol Cells were stimulated in quadruplicate with FhWey-ES (10 µg/ml for 24 hours) or FhWild-ES (10 µg/ml for 24 hours)
Growth protocol The cells were cultivated in DMEM (with 5% FCS, 2 mM glutamine, 100 units/ml of penicillin, 100 µg/ml streptomycin)
Extracted molecule total RNA
Extraction protocol total RNA was extracted using RNA isolation kit (A&A Biotechnology)
Label Cy3
Label protocol Quick Amp Labeling (Agilent Technologies) according to the manufacturer`s protocol
 
Channel 2
Source name BOMA, bovine macrophage cell line stimulated with FhWey-ESP
Organism Bos taurus
Characteristics agent: FhWey-ES
cell line: BOMA
cell type: monocytes/ macrophages
Treatment protocol Cells were stimulated in quadruplicate with FhWey-ES (10 µg/ml for 24 hours) or FhWild-ES (10 µg/ml for 24 hours)
Growth protocol The cells were cultivated in DMEM (with 5% FCS, 2 mM glutamine, 100 units/ml of penicillin, 100 µg/ml streptomycin)
Extracted molecule total RNA
Extraction protocol total RNA was extracted using RNA isolation kit (A&A Biotechnology)
Label Cy5
Label protocol Quick Amp Labeling (Agilent Technologies) according to the manufacturer`s protocol
 
 
Hybridization protocol according to the manufacturer's protocol
Scan protocol Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
Description Technical replicate 2 of 4
Data processing Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
 
Submission date Feb 17, 2017
Last update date Mar 13, 2018
Contact name Piotr Bąska
E-mail(s) piotr_baska@sggw.pl
Phone 48225936038
Organization name Warsaw University of Life Sciences WULS – SGGW
Department Department of Preclinical Sciences
Lab Division of Pharmacology and Toxicology
Street address Ciszewskiego 8
City Warsaw
State/province Mazowieckie
ZIP/Postal code 02-786
Country Poland
 
Platform ID GPL11648
Series (1)
GSE95019 Analysis of influence of Excretory Secretory Products from wild and laboratory Fasciola hepatica isolates on bovine macrophages

Data table header descriptions
ID_REF
VALUE normalized log10 ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
1 1.249544542e-003
2 -1.790536377e-001
3 0.000000000e+000
4 0.000000000e+000
5 0.000000000e+000
6 0.000000000e+000
7 0.000000000e+000
8 0.000000000e+000
9 -3.698000037e-001
10 0.000000000e+000
11 0.000000000e+000
12 1.099934041e-001
13 1.445413253e-002
14 -1.066907128e-001
15 -1.830665531e-001
16 0.000000000e+000
17 1.818231567e-002
18 -3.373816528e-001
19 -2.123136007e-001
20 -3.366628709e-001

Total number of rows: 45220

Table truncated, full table size 1023 Kbytes.




Supplementary file Size Download File type/resource
GSM2494619_BOMA_FhWey_ESP_rep_2.txt.gz 15.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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