NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2494631 Query DataSets for GSM2494631
Status Public on Mar 13, 2018
Title Bovine macrophages stimulated with FhWild-ES, replicate 4
Sample type RNA
 
Channel 1
Source name BOMA, bovine macrophage cell line
Organism Bos taurus
Characteristics cell line: BOMA
cell type: monocytes/ macrophages
agent: none
Treatment protocol Cells were stimulated in quadruplicate with FhWey-ES (10 µg/ml for 24 hours) or FhWild-ES (10 µg/ml for 24 hours)
Growth protocol The cells were cultivated in DMEM (with 5% FCS, 2 mM glutamine, 100 units/ml of penicillin, 100 µg/ml streptomycin)
Extracted molecule total RNA
Extraction protocol total RNA was extracted using RNA isolation kit (A&A Biotechnology)
Label Cy3
Label protocol Quick Amp Labeling (Agilent Technologies) according to the manufacturer`s protocol
 
Channel 2
Source name BOMA, bovine macrophage cell line stimulated with FhWild-ESP
Organism Bos taurus
Characteristics agent: FhWild-ES
cell line: BOMA
cell type: monocytes/ macrophages
Treatment protocol Cells were stimulated in quadruplicate with FhWey-ES (10 µg/ml for 24 hours) or FhWild-ES (10 µg/ml for 24 hours)
Growth protocol The cells were cultivated in DMEM (with 5% FCS, 2 mM glutamine, 100 units/ml of penicillin, 100 µg/ml streptomycin)
Extracted molecule total RNA
Extraction protocol total RNA was extracted using RNA isolation kit (A&A Biotechnology)
Label Cy5
Label protocol Quick Amp Labeling (Agilent Technologies) according to the manufacturer`s protocol
 
 
Hybridization protocol according to the manufacturer's protocol
Scan protocol Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
Description Technical replicate 4 of 4
Data processing Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
 
Submission date Feb 17, 2017
Last update date Mar 13, 2018
Contact name Piotr Bąska
E-mail(s) piotr_baska@sggw.edu.pl
Phone 48225936038
Organization name Warsaw University of Life Sciences WULS – SGGW
Department Department of Preclinical Sciences
Lab Division of Pharmacology and Toxicology
Street address Ciszewskiego 8
City Warsaw
State/province Mazowieckie
ZIP/Postal code 02-786
Country Poland
 
Platform ID GPL11648
Series (1)
GSE95019 Analysis of influence of Excretory Secretory Products from wild and laboratory Fasciola hepatica isolates on bovine macrophages

Data table header descriptions
ID_REF
VALUE normalized log10 ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
1 3.009265025e-002
2 0.000000000e+000
3 0.000000000e+000
4 0.000000000e+000
5 0.000000000e+000
6 0.000000000e+000
7 0.000000000e+000
8 0.000000000e+000
9 0.000000000e+000
10 0.000000000e+000
11 0.000000000e+000
12 4.612256248e-002
13 7.468064200e-002
14 8.207565730e-003
15 -9.865176893e-002
16 0.000000000e+000
17 -2.585062955e-002
18 0.000000000e+000
19 -3.217677133e-002
20 0.000000000e+000

Total number of rows: 45220

Table truncated, full table size 1023 Kbytes.




Supplementary file Size Download File type/resource
GSM2494631_BOMA_FhWild_ESP_rep_4.txt.gz 15.8 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap