|
Status |
Public on Mar 13, 2018 |
Title |
Bovine macrophages stimulated with FhWild-ES, replicate 4 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
BOMA, bovine macrophage cell line
|
Organism |
Bos taurus |
Characteristics |
cell line: BOMA cell type: monocytes/ macrophages agent: none
|
Treatment protocol |
Cells were stimulated in quadruplicate with FhWey-ES (10 µg/ml for 24 hours) or FhWild-ES (10 µg/ml for 24 hours)
|
Growth protocol |
The cells were cultivated in DMEM (with 5% FCS, 2 mM glutamine, 100 units/ml of penicillin, 100 µg/ml streptomycin)
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA was extracted using RNA isolation kit (A&A Biotechnology)
|
Label |
Cy3
|
Label protocol |
Quick Amp Labeling (Agilent Technologies) according to the manufacturer`s protocol
|
|
|
Channel 2 |
Source name |
BOMA, bovine macrophage cell line stimulated with FhWild-ESP
|
Organism |
Bos taurus |
Characteristics |
agent: FhWild-ES cell line: BOMA cell type: monocytes/ macrophages
|
Treatment protocol |
Cells were stimulated in quadruplicate with FhWey-ES (10 µg/ml for 24 hours) or FhWild-ES (10 µg/ml for 24 hours)
|
Growth protocol |
The cells were cultivated in DMEM (with 5% FCS, 2 mM glutamine, 100 units/ml of penicillin, 100 µg/ml streptomycin)
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA was extracted using RNA isolation kit (A&A Biotechnology)
|
Label |
Cy5
|
Label protocol |
Quick Amp Labeling (Agilent Technologies) according to the manufacturer`s protocol
|
|
|
|
Hybridization protocol |
according to the manufacturer's protocol
|
Scan protocol |
Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
Description |
Technical replicate 4 of 4
|
Data processing |
Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
|
|
Submission date |
Feb 17, 2017 |
Last update date |
Mar 13, 2018 |
Contact name |
Piotr Bąska |
E-mail(s) |
piotr_baska@sggw.edu.pl
|
Phone |
48225936038
|
Organization name |
Warsaw University of Life Sciences WULS – SGGW
|
Department |
Department of Preclinical Sciences
|
Lab |
Division of Pharmacology and Toxicology
|
Street address |
Ciszewskiego 8
|
City |
Warsaw |
State/province |
Mazowieckie |
ZIP/Postal code |
02-786 |
Country |
Poland |
|
|
Platform ID |
GPL11648 |
Series (1) |
GSE95019 |
Analysis of influence of Excretory Secretory Products from wild and laboratory Fasciola hepatica isolates on bovine macrophages |
|