|
| Status |
Public on Jan 08, 2019 |
| Title |
glp-4_NGM_B |
| Sample type |
RNA |
| |
|
| Source name |
glp-4, NGM (agar), 8h
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
media: NGM, E. coli OP50
treatment: None
tissue: Whole organism
|
| Treatment protocol |
Animals were grown until young adult stage. Afterward, worms were incubated for 12 hours at 25°C in 6-well plates with liquid S basal media + E. coli with media control or pyoverdine (PVD). A cohort of glp-4 worms that remaind on 10 cm NGM plates served as an additional control
|
| Growth protocol |
glp-4 worms were synchronized by hypochlorite treatment. Arrested L1s were plated on 10 cm NGM plates seeded with E. coli OP50 and grown at 15°C overnight then shifted to 25°C for 44h until they reached sterile young adult stage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRI Reagent (Molecular Research Center) according to manufacturer's instructions and purified using an RNeasy column (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Data processing |
Data were analyzed with GC-RMA in the R programming language, using the Bioconductor package gcrma. Analysis was performed using default settings.
|
| |
|
| Submission date |
Feb 28, 2017 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Natalia V Kirienko |
| E-mail(s) |
kirienko@rice.edu
|
| Organization name |
Rice University
|
| Department |
BioSciences
|
| Lab |
Kirienko
|
| Street address |
6100 Main St. MS140
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77005 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE95510 |
Translocation of Pyoverdine into Host Cells Mediates Iron Removal and Activates a Specific Host Immune Response |
|
