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Sample GSM2525646 Query DataSets for GSM2525646
Status Public on Dec 19, 2017
Title PBMC_Responder_Patient3_-3d
Sample type RNA
 
Source name peripheral blood
Organism Homo sapiens
Characteristics cell type: peripheral blood mononuclear cell
treatment response: responder
timepoint: -3 days
treatment: None
Treatment protocol RNA from peripheral blood mononuclear cell of 8 patients at 2 different time points (3 days before and 6 days after Sorafenib treatment) were extractied from 8 ml of blood.
Extracted molecule total RNA
Extraction protocol 2ug of total RNA from about 10^7 cells per sample were incubated with DNaseI according the Qiagen manufacture and cleaned up with RNeasy Micro Kit (Qiagen, Hilden, Germany).
Label Biotin
Label protocol RNA integrity was analyzed by capillary electrophoresis using a Fragment Analyser (Advanced Analytical Technologies, Inc. Ames, IA) and were further processed with the Affymetrix GeneChip Pico kit
 
Hybridization protocol Total RNA was hybridized to Affymetrix Clariom S arrays as described by the manufacturers (Affymetrix/Thermo Fisher Scientific, 168 Third Avenue, Waltham, MA USA 02451.)
Scan protocol Arrays were washed and stained with GeneChip Fluidics Station 450 and scanned with GeneChip Scanner 3000 using the GeneChip Operating Software (Affymetrix).
Description Gene expression data from PBMCs of a responder patient three days before Sorafenib treatment.
Data processing R/Bioconductor
 
Submission date Mar 07, 2017
Last update date Dec 19, 2017
Contact name Hauke Busch
E-mail(s) hauke.busch@uni-luebeck.de
Phone +49-451-3101-8470
Organization name University of Lübeck
Department Lübeck Institute of Experimental Dermatology
Street address Ratzeburger Allee 160
City Lübeck
State/province Schleswig-Holstein
ZIP/Postal code 23538
Country Germany
 
Platform ID GPL23159
Series (1)
GSE95770 Sorafenib promotes graft-versus-leukemia activity in mice and humans through IL-15 production in FLT3-ITD mutant leukemia cells

Data table header descriptions
ID_REF
VALUE gene level log2 RMA signal

Data table
ID_REF VALUE
TC0100006437.hg.1 5.984792772
TC0100006476.hg.1 7.588651919
TC0100006479.hg.1 7.936714367
TC0100006480.hg.1 7.290704321
TC0100006483.hg.1 8.488151905
TC0100006486.hg.1 7.679396389
TC0100006490.hg.1 5.694235106
TC0100006492.hg.1 7.13751226
TC0100006494.hg.1 6.261264945
TC0100006497.hg.1 6.779941652
TC0100006499.hg.1 7.048715367
TC0100006501.hg.1 6.285144552
TC0100006502.hg.1 6.168423554
TC0100006514.hg.1 7.222068311
TC0100006516.hg.1 7.110505547
TC0100006517.hg.1 4.769199607
TC0100006524.hg.1 7.56964245
TC0100006540.hg.1 5.937908024
TC0100006548.hg.1 4.633183287
TC0100006550.hg.1 6.531819484

Total number of rows: 21448

Table truncated, full table size 629 Kbytes.




Supplementary file Size Download File type/resource
GSM2525646_Responder3_minus3.CEL.gz 1.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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