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Sample GSM2526448 Query DataSets for GSM2526448
Status Public on Apr 03, 2017
Title Treated with acetic acid (9hr)
Sample type SRA
Source name whole plant
Organism Arabidopsis thaliana
Characteristics strain: C-0
time: 9h
treatment: acetic acid
antibody: Histone H4 ac antibody (Millipore 06-866)
Treatment protocol Treated with 10mM acetici acid solution or water for 9days. Solutions were added to soil.
Growth protocol Wild-type arabidopsis taliana (Col-0) were grown on soil for 14days.
Extracted molecule genomic DNA
Extraction protocol Please see Kim et al. (2014) Methods in Mol.Biol.;1062:405-26. doi: 10.1007/978-1-62703-580-4_22. ChIP assay was used Histon H4 ac antibody (Millipore 06-866).
Bar-code sequencing by SOLiD 5500 standard protocol (Thermo Fisher Scientific)
Used SOLiD 5500 standard protocol for ChIP-seq.
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model AB 5500xl Genetic Analyzer
Data processing Sequence fragments were aligned on TAIR10 genome using bowtie program (version 1.1.2).
Only pairs whose distance was less than 1kb were counted using our in-house program and peaks were identified using MACS2 program (version 2.1.1).
Genome_build: tair10
Supplementary_files_format_and_content: wiggle
Submission date Mar 08, 2017
Last update date May 15, 2019
Contact name Takaho A. Endo
Organization name RIKEN
Department IMS
Lab Laboratory for Integrative Genomics
Street address 1-7-22 Suehiro, Tsurumi
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platform ID GPL16033
Series (1)
GSE95817 Acetici acid treated A.thaliana (Col-0)
BioSample SAMN06547410
SRA SRX2620189

Supplementary file Size Download File type/resource
GSM2526448_A9_H4ac.wig.gz 3.2 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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