|
| Status |
Public on May 29, 2009 |
| Title |
Δrsf1 YPG30min biological-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
isogenic Δrsf1 mutant yeast grown to A600=0.6 in YPD then switched to YPG for 30 minutes
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: isogenic Δrsf1 mutant
|
| Treatment protocol |
Cells grown to A600 = 0.6 in 50 ml of rich medium containing dextrose as sole carbon source were collected by centrifugation, washed 2x in YPG and resupended in YPG prewarmed to 30ºC and incubated for the specified number of minutes
|
| Growth protocol |
Cells grown to A600 = 0.6 in 50 ml of rich medium containing dextrose, glycerol or ethanol as sole carbon source
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
total nucleic acids were extracted using the "hot phenol extraction" method (McEntee and Hudson, Anal Biochem. 1989 176(2):303-6.) PolyA mRNA was prepared according to oligotex manual specifications.
|
| Label |
biotin
|
| Label protocol |
1 µg ds cDNA was labeled according to Affymetrix manual specifications.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 µg of cRNA were hybridized for 16 hours at 37ºC. Fragmentation and hybridization were performed at the MSU Research Tecnhology Support
|
| Scan protocol |
Gene Chips were scanned in a high density GC3000 scanner using GCOS operating software
|
| Description |
Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S cerevisiae (submitted)
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0). Median of each chip scaled to 500, values less than 0.01 set to 0.01. MSU Research Tecnhology
|
| |
|
| Submission date |
Dec 27, 2007 |
| Last update date |
May 25, 2011 |
| Contact name |
George Gordon Roberts III |
| Organization name |
Wayne State University
|
| Department |
Immunology/Microbiology
|
| Lab |
Hudson
|
| Street address |
540 E. Canfield
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platform ID |
GPL90 |
| Series (1) |
| GSE10031 |
mRNA expression data in Δrsf1mutant during growth on, and transition to growth on glycerol as sole carbon source |
|
