|
| Status |
Public on Mar 18, 2009 |
| Title |
Rice overexpressed OsSKIPa, biological repeat 2 |
| Sample type |
RNA |
| |
|
| Source name |
Rice overexpressed OsSKIPa
|
| Organism |
Oryza sativa Japonica Group |
| Characteristics |
OsSKIPa over-xpressed plants
|
| Growth protocol |
Sterilized seeds of transgenic plants and wild type were germinated in MS medium. Positive transgenic plants were selected by adding 50 mM hygromycin for S58S, or 50 mM G418 for S59R into the medium. Ten-day-old uniform seedlings were transferred to hydroponics culture medium and 1-month-old whole plants were collected for microarray analysis. All transgenic plants were prechecked by real time RT-PCR for the expression level of OsSKIPa to confirm overexpression or suppression of the gene.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol(Invitrogen, Carlsbad, CA, USA) extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
GeneChips were scanned using the Affymetrix® GeneChip® Scanner 3000.
|
| Description |
shoots of S58S, biological rep2
S58S-2
|
| Data processing |
Affymetrix ® GeneChip® Operating SoftwareVersion1.0
|
| |
|
| Submission date |
Jan 02, 2008 |
| Last update date |
Mar 17, 2009 |
| Contact name |
Kabin Xie |
| E-mail(s) |
xiekabin@webmail.hzau.edu.cn
|
| Phone |
+86-27-87281716
|
| Fax |
+86-27-87297092
|
| URL |
http://www.ncpgr.cn/
|
| Organization name |
Huazhong Agriculture University
|
| Department |
National Key Laboratory of Crop Genetic Improvement
|
| Lab |
National Center of Plant Gene Research (Wuhan)
|
| Street address |
Shizi Shan Street
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE10054 |
Expression information of splicing factor OsSKIPa knock-down and overexpressed rice |
|
